ArticleScientific reports2015
Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- The Contribution of Native Protein Complexes to Targeted Protein Degradation.ACS chemical biology · 2026Article
- Specific proteolysis mediated by a p97-directed proteolysis-targeting chimera (p97-PROTAC).eLife · 2025Article
- A genome-wide CRISPR screen maps endogenous regulators of PPARG gene expression in bladder cancer.iScience · 2023Article
- Recent advances in therapeutic CRISPR-Cas9 genome editing: mechanisms and applications.Molecular biomedicine · 2023Review
- AnmicroPublication biology · 2022Article
- Expansion of the CRISPR/Cas Genome-Sculpting Toolbox: Innovations, Applications and Challenges.Molecular diagnosis & therapy · 2021Review
- Designing A Transgenic Chicken: Applying New Approaches toward A Promising Bioreactor.Cell journal · 2020Article
- Salt-inducible kinases (SIKs) regulate TGFβ-mediated transcriptional and apoptotic responses.Cell death & disease · 2020Article
- Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and β-Catenin-Independent Targets by Proteomics.Molecular cancer research : MCR · 2019Article
- FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning.EMBO reports · 2019Article
- Integral gene drives for population replacement.Biology open · 2019Article
- PathogenicWellcome open research · 2019Article
- Endogenous HIF2A reporter systems for high-throughput functional screening.Scientific reports · 2018Article
- Applications of genetically engineered human pluripotent stem cell reporters in cardiac stem cell biology.Current opinion in biotechnology · 2018Review
- The DUF1669 domain of FAM83 family proteins anchor casein kinase 1 isoforms.Science signaling · 2018Article
- PAWS1 controls Wnt signalling through association with casein kinase 1α.EMBO reports · 2018Article
- Brd4-Brd2 isoform switching coordinates pluripotent exit and Smad2-dependent lineage specification.EMBO reports · 2017Article
- Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time.Scientific reports · 2017Article
- Regulation of membrane ruffling by polarized STIM1 and ORAI1 in cortactin-rich domains.Scientific reports · 2017Article
- Quantitative Systems Biology to decipher design principles of a dynamic cell cycle network: the "Maximum Allowable mammalian Trade-Off-Weight" (MAmTOW).NPJ systems biology and applications · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high expression and sophisticated tools such as confocal microscopy and flow cytometry. Therefore, a simple, sensitive and robust transcriptional reporter system driven by endogenous promoter for studies into transcriptional regulation is desirable. We report a CRISPR/Cas9-based methodology for rapidly integrating a firefly luciferase gene in somatic cells under the control of endogenous promoter, using the TGFβ-responsive gene PAI-1. Our strategy employed a polycistronic cassette containing a non-fused GFP protein to ensure the detection of transgene delivery and rapid isolation of positive clones. We demonstrate that firefly luciferase cDNA can be efficiently delivered downstream of the promoter of the TGFβ-responsive gene PAI-1. Using chemical and genetic regulators of TGFβ signalling, we show that it mimics the transcriptional regulation of endogenous PAI-1 expression. Our unique approach has the potential to expedite studies on transcription of any gene in the context of its native chromatin landscape in somatic cells, allowing for robust high-throughput chemical and genetic screens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.