Evidence map›Paper›PMID 25922721›Full record

ArticleJournal of thoracic disease2015

Methylated APC and RASSF1A in multiple specimens contribute to the differential diagnosis of patients with undetermined solitary pulmonary nodules.

Li Gao, Erfu Xie, Tongfu Yu, Dan Chen, Lixia Zhang, Bingfeng Zhang, Fang Wang, Jian Xu, Peijun Huang, Xisheng Liu and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of thoracic disease, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 2 pooled it
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 2 syntheses or guidelines pooled it, 19 citations in OpenAlex.

  1. Clinical value of combinedOncology reviews · 2026
    Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Li Gao1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Erfu Xie1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Tongfu Yu1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Dan Chen1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Lixia Zhang1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Bingfeng Zhang1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Fang Wang1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Jian Xu1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Peijun Huang1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Xisheng Liu1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Bingliang Fang1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Shiyang Pan1 Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 2 National Key Clinical, Department of Laboratory Medicine, Nanjing 210029, China ; 3 Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China ; 4 Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Northern Jiangsu People's Hospital · CNNanjing Medical University · CNJiangsu Province Hospital · CNThe University of Texas MD Anderson Cancer Center · USThoracic Surgery Foundation · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundInactivation of tumor-suppressor gene (TSG) by promoter hypermethylation has been reported in many tumor types, including lung cancer. This study was designed to determine the methylated APC and RASSF1A genes in tumor tissue, serum and plasma of patients with early stage lung cancer.

methodsEighty-nine patients with undetermined solitary pulmonary nodules detected upon CT-scan were recruited in this study. DNA samples were extracted from biopsy tissues, serum and plasma and QMSP of APC and RASSF1A was carried out after bisulfite conversion. The 89 patients consist of 58 stage I lung cancer patients and 31 benign lung disease according to pathological report. Twenty-six cancer patients had matched biopsy tumor tissue, serum and plasma samples.

resultsThe methylation rates of APC and RASSF1A were 59.0% and 66.1% in biopsy tissues, 42.5% and 52.5% in serum, and 24.1% and 43.1% in plasma of cancer patients. For RASSF1A, different samples all showed a significant difference between cancer group and benign group (P<0.05). However, APC gene only explored the P value less than 0.05 in plasma result. Towards the 26 lung cancer patients with three matched samples, methylation rate in each sample type was more than 50.0% and displayed no difference.

conclusionsEvaluation of APC and RASSF1A promoter methylation by using QMSP appears to be very useful for the differential diagnosis of patients with undetermined solitary pulmonary nodules. Our results also suggested that plasma might be the best sample for clinical detection of early stage lung.

Indexed as

APCLung cancerquantitative methylation-specific PCRRASSF1A

Identifiers

PMID25922721
PMCPMC4387414
OpenAlexW2194166243

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.