ArticleJournal of experimental botany2015
Enhanced acetyl-CoA production is associated with increased triglyceride accumulation in the green alga Chlorella desiccata.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 91 citations in OpenAlex.
- Harnessing microalgae for bioproducts: innovations in synthetic biology.World journal of microbiology & biotechnology · 2025Review
- A multi-omic characterization of the physiological responses to salt stress in Scenedesmus obliquus UTEX393.Communications biology · 2025Article
- Unveiling the dual potential of microalgae and seaweed biomass for sustainable biofuel production: a review.RSC advances · 2025Review
- Iron rescues glucose-mediated photosynthesis repression during lipid accumulation in the green alga Chromochloris zofingiensis.Nature communications · 2024Article
- Increased triacylglycerol production in Rhodococcus opacus by overexpressing transcriptional regulators.Biotechnology for biofuels and bioproducts · 2024Article
- Creation of Environmentally Friendly Super "Dinitrotoluene Scavenger" Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Mechanisms of Sodium-Acetate-Induced DHA Accumulation in a DHA-Producing Microalga,Marine drugs · 2022Article
- ATP-citrate lyase promotes axonal transport across species.Nature communications · 2021Article
- Fatty Acid Production and Direct Acyl Transfer through Polar Lipids Control TAG Biosynthesis during Nitrogen Deprivation in the Halotolerant AlgaMarine drugs · 2021Article
- Quantitative proteomic comparison of salt stress in Chlamydomonas reinhardtii and the snow alga Chlamydomonas nivalis reveals mechanisms for salt-triggered fatty acid accumulation via reallocation of carbon resources.Biotechnology for biofuels · 2021Article
- The MicroalgaPlant physiology · 2020Article
- Long-chain acyl-CoA synthetase 2 is involved in seed oil production in Brassica napus.BMC plant biology · 2020Article
- Inhibition of DNA Methylation inFrontiers in genetics · 2020Article
- Branched-Chain Amino Acid Catabolism Impacts Triacylglycerol Homeostasis inPlant physiology · 2019Article
- A hypothesis about the origin of carotenoid lipid droplets in the green algae Dunaliella and Haematococcus.Planta · 2019Review
- MTCH2-mediated mitochondrial fusion drives exit from naïve pluripotency in embryonic stem cells.Nature communications · 2018Article
- The Ancient Phosphatidylinositol 3-Kinase Signaling System Is a Master Regulator of Energy and Carbon Metabolism in Algae.Plant physiology · 2018Article
- Genetic Engineering ofFrontiers in microbiology · 2018Article
- Triacylglycerol is produced from starch and polar lipids in the green alga Dunaliella tertiolecta.Journal of experimental botany · 2017Article
- Time-resolved transcriptome analysis and lipid pathway reconstruction of the oleaginous green microalgaBiotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Triglycerides (TAGs) from microalgae can be utilized as food supplements and for biodiesel production, but little is known about the regulation of their biosynthesis. This work aimed to test the relationship between acetyl-CoA (Ac-CoA) levels and TAG biosynthesis in green algae under nitrogen deprivation. A novel, highly sensitive liquid chromatography mass spectrometry (LC-MS/MS) technique enabled us to determine the levels of Ac-CoA, malonyl-CoA, and unacetylated (free) CoA in green microalgae. A comparative study of three algal species that differ in TAG accumulation levels shows that during N starvation, Ac-CoA levels rapidly rise, preceding TAG accumulation in all tested species. The levels of Ac-CoA in the high TAG accumulator Chlorella desiccata exceed the levels in the moderate TAG accumulators Dunaliella tertiolecta and Chlamydomonas reinhardtii. Similarly, malonyl-CoA and free CoA levels also increase, but to lower extents. Calculated cellular concentrations of Ac-CoA are far lower than reported K mAc-CoA values of plastidic Ac-CoA carboxylase (ptACCase) in plants. Transcript level analysis of plastidic pyruvate dehydrogenase (ptPDH), the major chloroplastic Ac-CoA producer, revealed rapid induction in parallel with Ac-CoA accumulation in C. desiccata, but not in D. tertiolecta or C. reinhardtii. It is proposed that the capacity to accumulate high TAG levels in green algae critically depends on their ability to divert carbon flow towards Ac-CoA. This requires elevation of the chloroplastic CoA pool level and enhancement of Ac-CoA biosynthesis. These conclusions may have important implications for future genetic manipulation to enhance TAG biosynthesis in green algae.
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