Evidence map›Paper›PMID 25921659›Full record

ArticleMolecular imaging and biology2015

In Vivo Tracking of Streptococcal Infections of Subcutaneous Origin in a Murine Model.

Richard W Davis, Heather Eggleston, Frances Johnson, Matthias Nahrendorf, Paul E Bock, Tiffany Peterson, Peter Panizzi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular imaging and biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Sugar Shock: ProbingFrontiers in microbiology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Richard W DavisDepartment of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, 4306 Walker Building, Auburn, AL, 36849, USA.
Heather EgglestonDepartment of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, 4306 Walker Building, Auburn, AL, 36849, USA.
Frances JohnsonDepartment of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, 4306 Walker Building, Auburn, AL, 36849, USA.
Matthias NahrendorfCenter for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Simches Research Building, 185 Cambridge St., Boston, MA, 02114, USA.
Paul E BockDepartment of Pathology, Microbiology, & Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
Tiffany PetersonDepartment of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, 4306 Walker Building, Auburn, AL, 36849, USA.
Peter PanizziDepartment of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, 4306 Walker Building, Auburn, AL, 36849, USA. panizzi@auburn.edu.
Auburn University · USMassachusetts General Hospital · USVanderbilt University Medical Center · USWalker (United States) · US

Funding

MOLECULAR MECHANISMS OF FIBRINOLYSISR01HL056181 · NHLBI · VANDERBILT UNIVERSITY · PI BOCK, PAUL E · 1996 to 2013
$3.9M
Random Shear Shuttle BAC Libraries for Antimicrobial Discovery from Soil MetagenoR44AI085840 · NIAID · LUCIGEN CORPORATION · PI LI, XING-CONG, LILES, MARK R · 2012 to 2014
$2.6M
Mechanism of Staphylocoagulase-activated Blood ClottingR01HL071544 · NHLBI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI BOCK, PAUL E, VERHAMME, INGRID M · 2003 to 2016
$1.8M
Pathogen specific imaging of endocarditisR01HL114477 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI NAHRENDORF, MATTHIAS, PANIZZI, PETER ROLF · 2013 to 2016
$1.7M
Pathobiology of Staphyloccocus areus EndocarditisR00HL094533 · NHLBI · AUBURN UNIVERSITY AT AUBURN · PI PANIZZI, PETER ROLF · 2011 to 2013
$719k
NHLBI NIH HHS R00 HL094533NHLBI NIH HHS R01 HL056181NHLBI NIH HHS R01 HL071544NHLBI NIH HHS R01 HL114477NIAID NIH HHS 2R44AI085840-02NIAID NIH HHS R44 AI085840
6 · The paper itself

Abstract

purposeGeneration of plasmin in vivo by Streptococcus pyogenes is thought to localize the active protease complexes to the pathogen surface to aid in tissue dissemination. Here, we chose to follow cutaneous streptococcal infections by the use of non-invasive bioluminescence imaging to determine if this pathogen can be followed by this approach and the extent of bacterial spread in the absence of canonical plasminogen activation by streptokinase. PROCEDURES: Mice were injected subcutaneously with either bioluminescent strains of streptococci, namely Xen20 and Xen10 or S. pyogenes ALAB49. Bioluminescence imaging was performed daily and results were correlated with microbiological and histological analyses.

resultsComparative analysis of chronologic non-invasive datasets indicated that Xen20 did not disseminate from the initial infection site. Contrary to this, microbiological and histological analyses of Xen20 mice for total bacterial burden indicated sepsis and widespread pathogen involvement.

conclusionsThe use of bioluminescence in microbe-based studies requires genomic and pathologic characterization to correlate imaging results with underlying pathology.

Indexed as

Disease Models, AnimalAnimalsMiceMice, Inbred C57BLSkinStreptococcal InfectionsStreptococcus pyogenesBioluminescenceNon-invasive imagingPlasminogenStreptococcus pyogenesStreptokinase

Identifiers

PMID25921659
PMCPMC4807872
OpenAlexW2050858819

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.