ArticleObesity (Silver Spring, Md.)2015
Direct conversion of human myoblasts into brown-like adipocytes by engineered super-active PPARγ.
Article in Obesity (Silver Spring, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.
- Thermogenic Differentiation of Human Adipocyte Precursors in Culture: A Systematic Review.Cells · 2025Pooled it
- Retinoid X receptor γ interacts with peroxisome proliferator-activated receptor-γ to promote browning during adipose tissue differentiation.Adipocyte · 2025Article
- Activation of skeletal carbohydrate-response element binding protein (ChREBP)-mediated de novo lipogenesis increases intramuscular fat content in chickens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- STK38 is a PPARγ-interacting protein promoting adipogenesis.Adipocyte · 2021Article
- The difference of castration timing of Korean Hanwoo bulls does not significantly affect the carcass characteristics.Journal of animal science and technology · 2021Article
- Controlling large Boolean networks with single-step perturbations.Bioinformatics (Oxford, England) · 2019Article
- Carotid baroreceptor stimulation in obese rats affects white and brown adipose tissues differently in metabolic protection.Journal of lipid research · 2019Article
- Chemical compound-based direct reprogramming for future clinical applications.Bioscience reports · 2018Review
- LIPG signaling promotes tumor initiation and metastasis of human basal-like triple-negative breast cancer.eLife · 2018Article
- Tumor-associated myoepithelial cells promote the invasive progression of ductal carcinomaThe Journal of biological chemistry · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
objectiveTo determine whether super-activation of PPARγ can reprogram human myoblasts into brown-like adipocytes and to establish a new cell model for browning research.
methodsTo enhance the PPARγ signaling, M3, the transactivation domain of MyoD, was fused to PPARγ. PPARγ and M3-PPARγ-lentiviral vectors were used to convert human myoblasts into adipocytes. Brown adipocyte markers of the reprogrammed adipocytes were assessed by qPCR and protein analyses. White adipocytes differentiated from subcutaneous stromal vascular cells and perithyroid brown fat tissues were used as references.
resultsIn transient transfections, M3-PPARγ had a stronger constitutive activity than PPARγ by reporter assay. Although the transduction of either PPARγ or M3-PPARγ induced adipogenesis in myoblasts, M3-PPARγ drastically induced the brown adipocyte markers of UCP1, CIDEA, and PRDM16 by 1,050, 2.4, and 5.0 fold, respectively and increased mitochondria contents by 4 fold, compared to PPARγ.
conclusionsSuper-activation of PPARγ can effectively convert human myoblasts into brown-like adipocytes and a new approach to derive brown-like adipocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.