ArticleTissue engineering. Part C, Methods2015
Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering.
Article in Tissue engineering. Part C, Methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 91 citations in OpenAlex.
- Meniscal repair: The current state and recent advances in augmentation.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Pooled it
- Integrated strategies in meniscus tissue engineering: from biomaterials to stem cell-driven regeneration.Frontiers in bioengineering and biotechnology · 2026Review
- Collagen II and decellularized hyaline cartilage scaffolds derived from bovine trachea differentially promote chondrogenic differentiation of mesenchymal stem cells and decrease secretion of angiogenic factors.Cell and tissue research · 2025Article
- Decellularisation of human meniscus tissue using sodium dodecyl sulphate (SDS): Preserving biomechanical integrity for scaffold-based meniscal repair.Journal of experimental orthopaedics · 2025Article
- Analysis of Extracellular ATP Distribution in the Intervertebral Disc.Annals of biomedical engineering · 2024Article
- Article
- Allografts for partial meniscus repair: anFrontiers in bioengineering and biotechnology · 2023Article
- Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs.Frontiers in veterinary science · 2023Article
- Optimizing artificial meniscus by mechanical stimulation of the chondrocyte-laden acellular meniscus using ad hoc bioreactor.Stem cell research & therapy · 2022Article
- Applications and prospects of different functional hydrogels in meniscus repair.Frontiers in bioengineering and biotechnology · 2022Review
- Natural biopolymer scaffold for meniscus tissue engineering.Frontiers in bioengineering and biotechnology · 2022Review
- Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis.Beilstein journal of nanotechnology · 2022Review
- Decellularized Extracellular Matrix as a Potent Natural Biomaterial for Regenerative Medicine.Advances in experimental medicine and biology · 2021Article
- Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Article
- Preparation and Characterization of an Optimized Meniscal Extracellular Matrix Scaffold for Meniscus Transplantation.Frontiers in bioengineering and biotechnology · 2020Article
- Meniscus-Derived Matrix Bioscaffolds: Effects of Concentration and Cross-Linking on Meniscus Cellular Responses and Tissue Repair.International journal of molecular sciences · 2019Article
- Decellularized Lymph Node Scaffolding as a Carrier for Dendritic Cells to Induce Anti-Tumor Immunity.Pharmaceutics · 2019Article
- Meniscus-Derived Matrix Scaffolds Promote the Integrative Repair of Meniscal Defects.Scientific reports · 2019Article
- Surface modification of decellularized trachea matrix with collagen and laser micropore technique to promote cartilage regeneration.American journal of translational research · 2019Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Given the growing number of arthritis patients and the limitations of current treatments, there is great urgency to explore cartilage substitutes by tissue engineering. In this study, we developed a novel decellularization method for menisci to prepare acellular extracellular matrix (ECM) scaffolds with minimal adverse effects on the ECM. Among all the acid treatments, formic acid treatment removed most of the cellular contents and preserved the highest ECM contents in the decellularized porcine menisci. Compared with fresh porcine menisci, the content of DNA decreased to 4.10%±0.03%, and there was no significant damage to glycosaminoglycan (GAG) or collagen. Histological staining also confirmed the presence of ECM and the absence of cellularity. In addition, a highly hydrophilic scaffold with three-dimensional interconnected porous structure was fabricated from decellularized menisci tissue. Human chondrocytes showed enhanced cell proliferation and synthesis of chondrocyte ECM including type II collagen and GAG when cultured in this acellular scaffold. Moreover, the scaffold effectively supported chondrogenesis of human bone marrow-derived mesenchymal stem cells. Finally, in vivo implantation was conducted in rats to assess the biocompatibility of the scaffolds. No significant inflammatory response was observed. The acellular ECM scaffold provided a native environment for cells with diverse physiological functions to promote cell proliferation and new tissue formation. This study reported a novel way to prepare decellularized meniscus tissue and demonstrated the potential as scaffolds to support cartilage repair.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.