ArticlePloS one2015
Exosomes are unlikely involved in intercellular Nef transfer.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- HIV-1 Nef is carried on the surface of extracellular vesicles.Journal of extracellular vesicles · 2024Article
- HIV Nef Expression Down-modulated GFAP Expression and Altered Glutamate Uptake and Release and Proliferation in Astrocytes.Aging and disease · 2023Article
- The Persistence of HIV Diversity, Transcription, and Nef Protein in Kaposi's Sarcoma Tumors during Antiretroviral Therapy.Viruses · 2022Article
- Exploiting Manipulated Small Extracellular Vesicles to Subvert Immunosuppression at the Tumor Microenvironment through Mannose Receptor/CD206 Targeting.International journal of molecular sciences · 2020Review
- Myosin-X is essential to the intercellular spread of HIV-1 Nef through tunneling nanotubes.Journal of cell communication and signaling · 2019Article
- Exosomes Released from Rabies Virus-Infected Cells May be Involved in the Infection Process.Virologica Sinica · 2019Article
- Extracellular vesicles and chronic inflammation during HIV infection.Journal of extracellular vesicles · 2019Review
- Nef-induced CCL2 Expression Contributes to HIV/SIV Brain Invasion and Neuronal Dysfunction.Frontiers in immunology · 2019Review
- Role of Exosomes in Human Retroviral Mediated Disorders.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2018Review
- Tetraspanin blockage reduces exosome-mediated HIV-1 entry.Archives of virology · 2018Article
- Article
- Consequence of HIV and HCV co-infection on host immune response, persistence and current treatment options.Virusdisease · 2018Review
- Article
- Viral protein Nef is detected in plasma of half of HIV-infected adults with undetectable plasma HIV RNA.PloS one · 2018Article
- Nef is secreted in exosomes from Nef.GFP-expressing and HIV-1-infected human astrocytes.Journal of neurovirology · 2017Article
- Viral effects on the content and function of extracellular vesicles.Nature reviews. Microbiology · 2017Review
- Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture.Scientific reports · 2017Article
- Extracellular vesicles of the blood-brain barrier: Role in the HIV-1 associated amyloid beta pathology.Molecular and cellular neurosciences · 2017Article
- Article
- HIV-1 Nef is released in extracellular vesicles derived from astrocytes: evidence for Nef-mediated neurotoxicity.Cell death & disease · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
HIV-1 Nef is an important pathogenic factor for HIV/AIDS pathogenesis. Several recent studies including ours have demonstrated that Nef can be transferred to neighboring cells and alters the function of these cells. However, how the intercellular Nef transfer occurs is in dispute. In the current study, we attempted to address this important issue using several complementary strategies, a panel of exosomal markers, and human CD4+ T lymphocyte cell line Jurkat and a commonly used cell line 293T. First, we showed that Nef was transferred from Nef-expressing or HIV-infected Jurkat to naïve Jurkat and other non-Jurkat cells and that the transfer required the membrane targeting function of Nef and was cell density-dependent. Then, we showed that Nef transfer was cell-cell contact-dependent, as exposure to culture supernatants or exosomes from HIV-infected Jurkat or Nef-expressing Jurkat and 293T led to little Nef detection in the target cells Jurkat. Thirdly, we demonstrated that Nef was only detected to be associated with HIV virions but not with acetylcholinesterase (AChE+) exosomes from HIV-infected Jurkat and not in the exosomes from Nef-expressing Jurkat. In comparison, when it was over-expressed in 293T, Nef was detected in detergent-insoluble AChE+/CD81 low/TSG101 low exosomes, but not in detergent-soluble AChE-/CD81 high/TSG101 high exosomes. Lastly, microscopic imaging showed no significant Nef detection in exosomal vesicle-like structures in and out 293T. Taken together, these results show that exosomes are unlikely involved in intercellular Nef transfer. In addition, this study reveals existence of two types of exosomes: AChE+/CD81 low/TSG101 low exosomes and AChE-/CD81 high/TSG101 high exosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.