ArticlePloS one2015
Dynamic Metabolic Profiles and Tissue-Specific Source Effects on the Metabolome of Developing Seeds of Brassica napus.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 36 citations in OpenAlex.
- Roles of Metabolites Unveiled by Metabolomics inMetabolites · 2026Review
- Metabolic mechanism of nitrogen modified atmosphere storage on delaying quality deterioration of rice grains.Food chemistry: X · 2022Article
- The Transcriptome and Metabolome Reveal the Potential Mechanism of Lodging Resistance in Intergeneric Hybrids betweenInternational journal of molecular sciences · 2022Article
- Metabolite Characteristics Analysis of Siliques and Effects of Lights on the Accumulation of Glucosinolates in Siliques of Rapeseed.Frontiers in plant science · 2022Article
- Transcriptomic comparison of seeds and silique walls from two rapeseed genotypes with contrasting seed oil content.Frontiers in plant science · 2022Article
- Light induces gene expression to enhance the synthesis of storage reserves in Brassica napus L. embryos.Plant molecular biology · 2020Article
- Article
- Correlation analysis of the transcriptome and metabolome reveals the regulatory network for lipid synthesis in developing Brassica napus embryos.Plant molecular biology · 2019Article
- Metabolomics analysis based on a UPLC-Q-TOF-MS metabolomics approach to compare Lin-Xia-Shan-Shen and garden ginseng.RSC advances · 2018Article
- Integration of omics approaches to understand oil/protein content during seed development in oilseed crops.Plant cell reports · 2017Review
- GC-MS Metabolomic Analysis to Reveal the Metabolites and Biological Pathways Involved in the Developmental Stages and Tissue Response of Panax ginseng.Molecules (Basel, Switzerland) · 2017Article
- Genetic effects and genotype × environment interactions govern seed oil content in Brassica napus L.BMC genetics · 2017Article
- Nutritional functions of the funiculus in Brassica napus seed maturation revealed by transcriptome and dynamic metabolite profile analyses.Plant molecular biology · 2016Article
- Metabolic Analysis of Medicinal Dendrobium officinale and Dendrobium huoshanense during Different Growth Years.PloS one · 2016Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Canola (Brassica napus) is one of several important oil-producing crops, and the physiological processes, enzymes, and genes involved in oil synthesis in canola seeds have been well characterized. However, relatively little is known about the dynamic metabolic changes that occur during oil accumulation in seeds, as well as the mechanistic origins of metabolic changes. To explore the metabolic changes that occur during oil accumulation, we isolated metabolites from both seed and silique wall and identified and characterized them by using gas chromatography coupled with mass spectrometry (GC-MS). The results showed that a total of 443 metabolites were identified from four developmental stages. Dozens of these metabolites were differentially expressed during seed ripening, including 20 known to be involved in seed development. To investigate the contribution of tissue-specific carbon sources to the biosynthesis of these metabolites, we examined the metabolic changes of silique walls and seeds under three treatments: leaf-detachment (Ld), phloem-peeling (Pe), and selective silique darkening (Sd). Our study demonstrated that the oil content was independent of leaf photosynthesis and phloem transport during oil accumulation, but required the metabolic influx from the silique wall. Notably, Sd treatment resulted in seed senescence, which eventually led to a severe reduction of the oil content. Sd treatment also caused a significant accumulation of fatty acids (FA), organic acids and amino acids. Furthermore, an unexpected accumulation of sugar derivatives and organic acid was observed in the Pe- and Sd-treated seeds. Consistent with this, the expression of a subset of genes involved in FA metabolism, sugar and oil storage was significantly altered in Pe and Sd treated seeds. Taken together, our studies suggest the metabolite profiles of canola seeds dynamically varied during the course of oil accumulation, which may provide a new insight into the mechanisms of the oil accumulation at the metabolite level.
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