Evidence map›Paper›PMID 25919337›Full record

ArticleMicrobial biotechnology2015

Protein transduction domain of transactivating transcriptional activator fused to outer membrane protein K of Vibrio parahaemolyticus to vaccinate marbled eels (Anguilla marmorata) confers protection against mortality caused by V. parahaemolyticus.

Hang Wang, Wei Yang, Guoying Shen, Jianting Zhang, Wei Lv, Binfeng Ji, Chun Meng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Hang WangInstitute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Wei YangInstitute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Guoying ShenInstitute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Jianting ZhangInstitute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Wei LvInstitute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Binfeng JiInstitute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Chun MengInstitute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350002, China.
Fuzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although immersion and oral vaccination are the most practical methods for fish farmers, their applications are very limited due to low immune stimulation effect. We used the protein transduction domain (PTD) of transactivating transcriptional factor (TAT) derived from HIV TAT protein to increase the delivery efficiency of aquatic protein vaccines. Vibrio parahaemolyticus outer membrane protein K (ompK), a reported vaccine candidate for the prevention of V. parahaemolyticus infection, was fused with TAT-PTD expressed in Escherichia coli. We found that PTD-ompK fusion protein effectively penetrated into marbled eel bodies. Analysis of ompK antibody titres demonstrated that immersion vaccination with PTD-ompK was superior to ompK alone and induced robust immune stimulation in marbled eels. Both active and passive protection analyses against immersive challenge with V. parahaemolyticus strains demonstrated that marbled eels immunized with PTD-ompK survived significantly longer than those immunized with ompK alone. Our results indicated that TAT-PTD could be served as is an efficient delivery system for aquatic immersion vaccinations against various infectious diseases commonly seen in aquatic farm industry.

Indexed as

AnguillaAnimalsAntigens, BacterialBacterial Outer Membrane ProteinsBacterial VaccinesEscherichia coliGene ExpressionRecombinant Fusion ProteinsSurvival Analysistat Gene Products, Human Immunodeficiency VirusTrans-ActivatorsTreatment OutcomeVaccines, SyntheticVibrio InfectionsVibrio parahaemolyticusAntigens, BacterialBacterial Outer Membrane ProteinsBacterial VaccinesRecombinant Fusion Proteinstat Gene Products, Human Immunodeficiency VirusTrans-ActivatorsVaccines, Synthetic

Identifiers

PMID25919337
PMCPMC4476822
OpenAlexW1555498599

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.