ArticleMetabolites2015
Fermentative production of the diamine putrescine: system metabolic engineering of corynebacterium glutamicum.
Article in Metabolites, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 77 citations in OpenAlex.
- Enhancement and balance of 1,4-butanediamine biosynthesis in Escherichia coli based on modular metabolic engineering.Journal, genetic engineering & biotechnology · 2026Article
- Cisternostomy Facilitates Clearance of Metabolic Waste from Cerebrospinal Fluid in Patients with Severe Brain Injury.Aging and disease · 2025Article
- Review of the Proteomics and Metabolic Properties ofMicroorganisms · 2024Review
- Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers.Chemical reviews · 2023Review
- Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor inBioengineering (Basel, Switzerland) · 2023Article
- High-efficiency production of 5-hydroxyectoine using metabolically engineered Corynebacterium glutamicum.Microbial cell factories · 2022Article
- Article
- Metabolic Engineering ofMicroorganisms · 2022Article
- Production of l-glutamate family amino acids inSynthetic and systems biotechnology · 2021Review
- Adaptive laboratory evolution accelerated glutarate production by Corynebacterium glutamicum.Microbial cell factories · 2021Article
- Recent progress in production of amino acid-derived chemicals using Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2021Review
- The Effect of Halloysite Nanotubes on the Fire Retardancy Properties of Partially Biobased Polyamide 610.Polymers · 2020Article
- Diamine Biosynthesis: Research Progress and Application Prospects.Applied and environmental microbiology · 2020Review
- Inorganic Phosphate Solubilization by Rhizosphere BacteriumFrontiers in microbiology · 2020Article
- Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids.Nature communications · 2019Article
- Biological conversion of methane to putrescine using genome-scale model-guided metabolic engineering of a methanotrophic bacteriumBiotechnology for biofuels · 2019Article
- EngineeringBiotechnology for biofuels · 2019Article
- Function of L-Pipecolic Acid as Compatible Solute inFrontiers in microbiology · 2019Article
- Optimization of ʟ-ornithine production in recombinant Corynebacterium glutamicum S9114 by cg3035 overexpression and manipulating the central metabolic pathway.Microbial cell factories · 2018Article
- Pathway engineering in3 Biotech · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Corynebacterium glutamicum shows great potential for the production of the glutamate-derived diamine putrescine, a monomeric compound of polyamides. A genome-scale stoichiometric model of a C. glutamicum strain with reduced ornithine transcarbamoylase activity, derepressed arginine biosynthesis, and an anabolic plasmid-addiction system for heterologous expression of E. coli ornithine decarboxylase gene speC was investigated by flux balance analysis with respect to its putrescine production potential. Based on these simulations, enhancing glycolysis and anaplerosis by plasmid-borne overexpression of the genes for glyceraldehyde 3-phosphate dehydrogenase and pyruvate carboxylase as well as reducing 2-oxoglutarate dehydrogenase activity were chosen as targets for metabolic engineering. Changing the translational start codon of the chromosomal gene for 2-oxoglutarate dehydrogenase subunit E1o to the less preferred TTG and changing threonine 15 of OdhI to alanine reduced 2-oxoglutarate dehydrogenase activity about five fold and improved putrescine titers by 28%. Additional engineering steps improved further putrescine production with the largest contributions from preventing the formation of the by-product N-acetylputrescine by deletion of spermi(di)ne N-acetyltransferase gene snaA and from overexpression of the gene for a feedback-resistant N-acetylglutamate kinase variant. The resulting C. glutamicum strain NA6 obtained by systems metabolic engineering accumulated two fold more putrescine than the base strain, i.e., 58.1 ± 0.2 mM, and showed a specific productivity of 0.045 g·g-1·h-1 and a yield on glucose of 0.26 g·g-1.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.