ArticleProceedings of the National Academy of Sciences of the United States of America2015
Inverted formin 2 in focal adhesions promotes dorsal stress fiber and fibrillar adhesion formation to drive extracellular matrix assembly.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Diffusion model predicts the geometry of actin cytoskeleton from cell morphology.PLoS computational biology · 2024Article
- Ultrastructure of the actin cytoskeleton.Current opinion in cell biology · 2018Review
- Coordination of microtubule acetylation and the actin cytoskeleton by formins.Cellular and molecular life sciences : CMLS · 2018Review
- The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation viaThe Journal of cell biology · 2018Article
- Integrin-bound talin head inhibits actin filament barbed-end elongation.The Journal of biological chemistry · 2018Article
- Inverted formins: A subfamily of atypical formins.Cytoskeleton (Hoboken, N.J.) · 2017Review
- A cytoskeletal clutch mediates cellular force transmission in a soft, three-dimensional extracellular matrix.Molecular biology of the cell · 2017Article
- Dissipation of contractile forces: the missing piece in cell mechanics.Molecular biology of the cell · 2017Article
- Actin-Based Adhesion Modules Mediate Cell Interactions with the Extracellular Matrix and Neighboring Cells.Cold Spring Harbor perspectives in biology · 2017Review
- An integrated enhancement and reconstruction strategy for the quantitative extraction of actin stress fibers from fluorescence micrographs.BMC bioinformatics · 2017Article
- The assembly and function of perinuclear actin cap in migrating cells.Protoplasma · 2017Review
- Dysregulation of INF2-mediated mitochondrial fission in SPOP-mutated prostate cancer.PLoS genetics · 2017Article
- Tropomyosin Isoforms Specify Functionally Distinct Actin Filament Populations In Vitro.Current biology : CB · 2017Article
- Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA.Journal of cell science · 2017Article
- FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis.Cell · 2016Article
- Function and regulation of the Arp2/3 complex during cell migration in diverse environments.Current opinion in cell biology · 2016Review
- Tropomyosin Promotes Lamellipodial Persistence by Collaborating with Arp2/3 at the Leading Edge.Current biology : CB · 2016Article
- Actomyosin stress fiber mechanosensing in 2D and 3D.F1000Research · 2016Review
- Article
- Specification of Architecture and Function of Actin Structures by Actin Nucleation Factors.Annual review of biophysics · 2015Review
Corrections and comments
- Retraction · 2018-03-12Falsification/Fabrication of Data · Falsification/Fabrication of Image · Investigation by Company/Institution · Investigation by ORI · Misconduct - Official Investigation(s) and/or Finding(s) · Misconduct by Author · · Colleen Skau has NIH finding of misconduct: https://www.ncbi.nlm.nih.gov/pubmed/29493659
- Commented on by
- Retracted
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Actin filaments and integrin-based focal adhesions (FAs) form integrated systems that mediate dynamic cell interactions with their environment or other cells during migration, the immune response, and tissue morphogenesis. How adhesion-associated actin structures obtain their functional specificity is unclear. Here we show that the formin-family actin nucleator, inverted formin 2 (INF2), localizes specifically to FAs and dorsal stress fibers (SFs) in fibroblasts. High-resolution fluorescence microscopy and manipulation of INF2 levels in cells indicate that INF2 plays a critical role at the SF-FA junction by promoting actin polymerization via free barbed end generation and centripetal elongation of an FA-associated actin bundle to form dorsal SF. INF2 assembles into FAs during maturation rather than during their initial generation, and once there, acts to promote rapid FA elongation and maturation into tensin-containing fibrillar FAs in the cell center. We show that INF2 is required for fibroblasts to organize fibronectin into matrix fibers and ultimately 3D matrices. Collectively our results indicate an important role for the formin INF2 in specifying the function of fibrillar FAs through its ability to generate dorsal SFs. Thus, dorsal SFs and fibrillar FAs form a specific class of integrated adhesion-associated actin structure in fibroblasts that mediates generation and remodeling of ECM.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.