ArticleMolecular and cellular biology2015
Argonaute 2 Binds Directly to tRNA Genes and Promotes Gene Repression in cis.
Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Centrosome‑, mitotic spindle‑ and cytokinetic bridge‑specific compartmentalization of AGO2 protein in human liver cells undergoing mitosis: Non‑canonical, RNAi‑dependent, control of local homeostasis.Molecular medicine reports · 2025Article
- Culture conditions greatly impact the levels of vesicular and extravesicular Ago2 and RNA in extracellular vesicle preparations.Journal of extracellular vesicles · 2023Article
- Toward an Understanding of Extracellular tRNA Biology.Frontiers in molecular biosciences · 2021Article
- MAF1 is a chronic repressor of RNA polymerase III transcription in the mouse.Scientific reports · 2020Article
- Transcriptional repression of Myc underlies the tumour suppressor function of AGO1 inDevelopment (Cambridge, England) · 2020Article
- The prognostic value of microRNA-biogenesis genesAmerican journal of translational research · 2020Article
- Alanine tRNAs Translate Environment Into Behavior inFrontiers in cell and developmental biology · 2020Article
- Argonaute2 attenuates active transcription by limiting RNA Polymerase II elongation in Drosophila melanogaster.Scientific reports · 2018Article
- Maf1 and Repression of RNA Polymerase III-Mediated Transcription Drive Adipocyte Differentiation.Cell reports · 2018Article
- DUSP11 - An RNA phosphatase that regulates host and viral non-coding RNAs in mammalian cells.RNA biology · 2017Article
- Aggregation of Mod5 is affected by tRNA binding with implications for tRNA gene-mediated silencing.FEBS letters · 2017Article
- DUSP11 activity on triphosphorylated transcripts promotes Argonaute association with noncanonical viral microRNAs and regulates steady-state levels of cellular noncoding RNAs.Genes & development · 2016Article
- Human Argonaute 2 Is Tethered to Ribosomal RNA through MicroRNA Interactions.The Journal of biological chemistry · 2016Article
- RNA Polymerase III Advances: Structural and tRNA Functional Views.Trends in biochemical sciences · 2016Review
- Regulation of mammalian transcription and splicing by Nuclear RNAi.Nucleic acids research · 2016Review
- The Regulation of rRNA Gene Transcription during Directed Differentiation of Human Embryonic Stem Cells.PloS one · 2016Article
- Human Ribosomal RNA-Derived Resident MicroRNAs as the Transmitter of Information upon the Cytoplasmic Cancer Stress.BioMed research international · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To further our understanding of the RNAi machinery within the human nucleus, we analyzed the chromatin and RNA binding of Argonaute 2 (AGO2) within human cancer cell lines. Our data indicated that AGO2 binds directly to nascent tRNA and 5S rRNA, and to the genomic loci from which these RNAs are transcribed, in a small RNA- and DICER-independent manner. AGO2 chromatin binding was not observed at non-TFIIIC-dependent RNA polymerase III (Pol III) genes or at extra-TFIIIC (ETC) sites, indicating that the interaction is specific for TFIIIC-dependent Pol III genes. A genome-wide analysis indicated that loss of AGO2 caused a global increase in mRNA expression level among genes that flank AGO2-bound tRNA genes. This effect was shown to be distinct from that of the disruption of DICER, DROSHA, or CTCF. We propose that AGO2 binding to tRNA genes has a novel and important regulatory role in human cells.
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