ArticleThe Journal of cell biology2015
Myosin light chain kinase regulates cell polarization independently of membrane tension or Rho kinase.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Single-cell chiral symmetry breaking under confinement.bioRxiv : the preprint server for biology · 2026Article
- Modeling actin-microtubule crosstalk in migrating cells.Biophysical journal · 2025Article
- Galvanin (TMEM154) is an electric-field sensor for directed cell migration.bioRxiv : the preprint server for biology · 2025Article
- Excitable Rho dynamics control cell shape and motility by sequentially activating ERM proteins and actomyosin contractility.Science advances · 2024Article
- Article
- Next-generation plasmids for transgenesis in zebrafish and beyond.Development (Cambridge, England) · 2023Article
- Wound Healing Insights from Flies and Fish.Cold Spring Harbor perspectives in biology · 2022Review
- Leading edge maintenance in migrating cells is an emergent property of branched actin network growth.eLife · 2022Article
- Wounding Zebrafish Larval Epidermis by Laceration.Bio-protocol · 2021Article
- Comparative mapping of crawling-cell morphodynamics in deep learning-based feature space.PLoS computational biology · 2021Article
- Elastic wrinkling of keratocyte lamellipodia driven by myosin-induced contractile stress.Biophysical journal · 2021Article
- Osmolarity-independent electrical cues guide rapid response to injury in zebrafish epidermis.eLife · 2020Article
- CompuCell3D Simulations Reproduce Mesenchymal Cell Migration on Flat Substrates.Biophysical journal · 2020Article
- Article
- A Tale of Two States: Normal and Transformed, With and Without Rigidity Sensing.Annual review of cell and developmental biology · 2019Review
- Apical-basal polarity inhibits epithelial-mesenchymal transition and tumour metastasis by PAR-complex-mediated SNAI1 degradation.Nature cell biology · 2019Article
- Steps in Mechanotransduction Pathways that Control Cell Morphology.Annual review of physiology · 2019Review
- A Salutary Role of Reactive Oxygen Species in Intercellular Tunnel-Mediated Communication.Frontiers in cell and developmental biology · 2018Review
- Adhesion-Dependent Wave Generation in Crawling Cells.Current biology : CB · 2017Article
- Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells.Nature cell biology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Cells polarize to a single front and rear to achieve rapid actin-based motility, but the mechanisms preventing the formation of multiple fronts are unclear. We developed embryonic zebrafish keratocytes as a model system for investigating establishment of a single axis. We observed that, although keratocytes from 2 d postfertilization (dpf) embryos resembled canonical fan-shaped keratocytes, keratocytes from 4 dpf embryos often formed multiple protrusions despite unchanged membrane tension. Using genomic, genetic, and pharmacological approaches, we determined that the multiple-protrusion phenotype was primarily due to increased myosin light chain kinase (MLCK) expression. MLCK activity influences cell polarity by increasing myosin accumulation in lamellipodia, which locally decreases protrusion lifetime, limiting lamellipodial size and allowing for multiple protrusions to coexist within the context of membrane tension limiting protrusion globally. In contrast, Rho kinase (ROCK) regulates myosin accumulation at the cell rear and does not determine protrusion size. These results suggest a novel MLCK-specific mechanism for controlling cell polarity via regulation of myosin activity in protrusions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.