ArticleJournal of bacteriology2015
Role and Function of LitR, an Adenosyl B12-Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Methanotroph Methylotuvimicrobium alcaliphilum 20Z-3E as a fumarate producer: transcriptomic analysis and the role of malic enzyme.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- A genome-scale metabolic reconstruction provides insight into the metabolism of the thermophilic bacterium Rhodothermus marinus.FEMS microbiology ecology · 2025Article
- Light inducible gene expression system for Streptomyces.Scientific reports · 2024Article
- Redox driven BNature communications · 2023Article
- Class II LitR serves as an effector of "short" LOV-type blue-light photoreceptor in Pseudomonas mendocina.Scientific reports · 2022Article
- Article
- Chromogenicity of aerobic spore-forming bacteria of the Bacillaceae family isolated from different ecological niches and physiographic zones.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Engineering Light-Control in Biology.Frontiers in bioengineering and biotechnology · 2022Review
- Biogeographic and Evolutionary Patterns of Trace Element Utilization in Marine Microbial World.Genomics, proteomics & bioinformatics · 2021Article
- Bioluminescence and Photoreception in Unicellular Organisms: Light-Signalling in a Bio-Communication Perspective.International journal of molecular sciences · 2021Review
- Light-Triggered Carotenogenesis inMicroorganisms · 2021Review
- Controlled Transcription of Regulator GeneMicroorganisms · 2020Article
- Light Response ofJournal of bacteriology · 2020Article
- Bioinformatics of Metalloproteins and Metalloproteomes.Molecules (Basel, Switzerland) · 2020Review
- Light-inducible carotenoid production controlled by a MarR-type regulator in Corynebacterium glutamicum.Scientific reports · 2019Article
- Mutational loss of carotenoids in alkaliphilicMicrobiology (Reading, England) · 2019Article
- Role and Function of Class III LitR, a Photosensor Homolog from Burkholderia multivorans.Journal of bacteriology · 2018Article
- Plasticity in oligomerization, operator architecture, and DNA binding in the mode of action of a bacterial BThe Journal of biological chemistry · 2018Article
- A New Facet of Vitamin BAnnual review of biochemistry · 2017Review
- Green-Light-Induced Inactivation of Receptor Signaling Using Cobalamin-Binding Domains.Angewandte Chemie (International ed. in English) · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledThe LitR/CarH family of proteins is a light-sensitive MerR family of transcriptional regulators that contain an adenosyl B12 (coenzyme B12 or AdoB12)-binding domain at the C terminus. The genes encoding these proteins are found in phylogenetically diverse bacterial genera; however, the biochemical properties of these proteins from Gram-positive bacteria remain poorly understood. We performed genetic and biochemical analyses of a homolog of the LitR protein from Bacillus megaterium QM B1551, a Gram-positive endospore-forming soil bacterium. Carotenoid production was induced by illumination in this bacterium. In vivo analysis demonstrated that LitR plays a central role in light-inducible carotenoid production and serves as a negative regulator of the light-inducible transcription of crt and litR itself. Biochemical evidence showed that LitR in complex with AdoB12 binds to the promoter regions of litR and the crt operon in a light-sensitive manner. In vitro transcription experiments demonstrated that AdoB12-LitR inhibited the specific transcription of the crt promoter generated by a σ(A)-containing RNA polymerase holoenzyme under dark conditions. Collectively, these data indicate that the AdoB12-LitR complex serves as a photoreceptor with DNA-binding activity in B. megaterium QM B1551 and that its function as a transcriptional repressor is fundamental to the light-induced carotenoid production. IMPORTANCE: Members of the LitR/CarH family are AdoB12-based photosensors involved in light-inducible carotenoid production in nonphototrophic Gram-negative bacteria. Our study revealed that Bacillus LitR in complex with AdoB12 also serves as a transcriptional regulator with a photosensory function, which indicates that the LitR/CarH family is generally involved in the light-inducible carotenoid production of nonphototrophic bacteria.
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