ArticleFood and environmental virology2015
Human Adenovirus Diversity in Water Samples Using a Next-Generation Amplicon Sequencing Approach.
Article in Food and environmental virology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Wastewater monitoring allows the detection of uncommon and highly pathogenic enterovirus types.Applied and environmental microbiology · 2025Article
- Characterization of Human Viral Diversity and Adenovirus Isolates in a Sewage Treatment Plant in Tianjin: Implications for Public Health and Advanced Monitoring.Food and environmental virology · 2025Article
- Article
- Circulation of adenovirus and other viruses in urban drainage channels: an environmental surveillance in Belém, Amazon region, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area.Microorganisms · 2022Article
- Prevalence, Distribution, and Genotypes of Adenovirus and Norovirus in the Puzi River and Its Tributaries and the Surrounding Areas in Taiwan.GeoHealth · 2021Article
- NGS Techniques Reveal a High Diversity of RNA Viral Pathogens and Papillomaviruses in Fresh Produce and Irrigation Water.Foods (Basel, Switzerland) · 2021Article
- Occurrence of Human Enteric Viruses in Water Sources and Shellfish: A Focus on Africa.Food and environmental virology · 2021Review
- SARS-CoV-2 in environmental perspective: Occurrence, persistence, surveillance, inactivation and challenges.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
- Viral indicators for tracking domestic wastewater contamination in the aquatic environment.Water research · 2020Review
- Elucidating cryptic dynamics ofEcology and evolution · 2020Article
- Detection of Human Enteric Viruses in French Polynesian Wastewaters, Environmental Waters and Giant Clams.Food and environmental virology · 2019Article
- Comprehensive Study on Enteric Viruses and Indicators in Surface Water in Kyoto, Japan, During 2014-2015 Season.Food and environmental virology · 2018Article
- Quantification of human adenovirus and norovirus in river water in the north-east of France.Environmental science and pollution research international · 2018Article
- Review
- Molecular epidemiology of human adenovirus infections in Denmark, 2011-2016.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018Article
- Caffeine levels as a predictor of Human mastadenovirus presence in surface waters-a case study in the Sinos River basin-Brazil.Environmental science and pollution research international · 2018Article
- Emerging recombinant noroviruses identified by clinical and waste water screening.Emerging microbes & infections · 2018Article
- Detection by Direct Next Generation Sequencing Analysis of Emerging Enterovirus D68 and C109 Strains in an Environmental Sample From Scotland.Frontiers in microbiology · 2018Article
- First detection of enteric adenoviruses genotype 41 in recreation spring areas of Taiwan.Environmental science and pollution research international · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aims to establish a straightforward and original workflow for high-throughput typing of human adenoviruses (HAdVs) in environmental samples. Occurrence of HAdVs in water is well documented worldwide, but data on diversity of HAdV types circulating in water are scarcely available. Here, the characterisation of viral particles was performed by determination of amplicon sequences using a next-generation sequencing (NGS) approach. Adenoviral DNA was either directly isolated from wastewater or river water concentrates or after a cell culture passage. Genome amplification targeted a hyper variable region of the hexon gene, allowing the discrimination of the 54 human adenoviral types described until now. After read generation on the benchtop MiSeq platform (Illumina), data were analysed using the Mothur software for identification of all HAdV species and types simultaneously present in a unique sample. NGS results showed a relatively wide HAdV diversity of up to six types in one sample, whereas Sanger sequencing always only retrieved the dominant one. Detected types included HAdV-1, HAdV-2, HAdV-3, HAdV-6, HAdV-12, HAdV-31, HAdV-40 and HAdV-41, HAdV-41 being the most abundant in tested samples. In addition, the influence of the cell line (A549 vs 293A cells) on the infectious HAdV typing results was clearly determined. The 293A appeared to be the most suitable cell line allowing the detection of a larger diversity of infectious HAdVs and reflecting a more realistic initial species distribution than using the A549 cells. These findings demonstrated the feasibility of amplicon sequencing NGS approach to identify viruses in complex environmental water samples.
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Registered trials
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