ArticleNucleic acids research2015
Stable complex formation of CENP-B with the CENP-A nucleosome.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Models for the architecture of the human inner kinetochore on centromeric α-satellite CENP-A nucleosome arrays.Nature communications · 2026Article
- Maternal CENP-C restores centromere symmetry in mammalian zygotes to ensure proper chromosome segregation.Developmental cell · 2026Article
- Preserving centromere identity: right amounts of CENP-A at the right place and time.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Independence of centromeric and pericentromeric chromatin stability on CCAN components.Molecular biology of the cell · 2025Article
- Molecular evolution of the mammalian kinetochore complex.bioRxiv : the preprint server for biology · 2024Article
- SETDB2 interacts with BUBR1 to induce accurate chromosome segregation independently of its histone methyltransferase activity.FEBS open bio · 2024Article
- CENP-A and CENP-B collaborate to create an open centromeric chromatin state.Nature communications · 2023Article
- Article
- CENP-A: A Histone H3 Variant with Key Roles in Centromere Architecture in Healthy and Diseased States.Results and problems in cell differentiation · 2022Article
- FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability.Frontiers in cell and developmental biology · 2022Article
- Structural studies of functional nucleosome complexes with transacting factors.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2022Review
- Diverse mechanisms of centromere specification.Current biology : CB · 2021Review
- Kinetochore assembly throughout the cell cycle.Seminars in cell & developmental biology · 2021Article
- Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma.Journal of Cancer · 2021Article
- A genetic memory initiates the epigenetic loop necessary to preserve centromere position.The EMBO journal · 2020Article
- Stable inheritance of CENP-A chromatin: Inner strength versus dynamic control.The Journal of cell biology · 2020Article
- CENP-A nucleosome-a chromatin-embedded pedestal for the centromere: lessons learned from structural biology.Essays in biochemistry · 2020Review
- CENP-B creates alternative epigenetic chromatin states permissive for CENP-A or heterochromatin assembly.Journal of cell science · 2020Article
- Review
- Bioinformatics Analysis Reveals Biomarkers With Cancer Stem Cell Characteristics in Lung Squamous Cell Carcinoma.Frontiers in genetics · 2020Article
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CENP-A and CENP-B are major components of centromeric chromatin. CENP-A is the histone H3 variant, which forms the centromere-specific nucleosome. CENP-B specifically binds to the CENP-B box DNA sequence on the centromere-specific repetitive DNA. In the present study, we found that the CENP-A nucleosome more stably retains human CENP-B than the H3.1 nucleosome in vitro. Specifically, CENP-B forms a stable complex with the CENP-A nucleosome, when the CENP-B box sequence is located at the proximal edge of the nucleosome. Surprisingly, the CENP-B binding was weaker when the CENP-B box sequence was located in the distal linker region of the nucleosome. This difference in CENP-B binding, depending on the CENP-B box location, was not observed with the H3.1 nucleosome. Consistently, we found that the DNA-binding domain of CENP-B specifically interacted with the CENP-A-H4 complex, but not with the H3.1-H4 complex, in vitro. These results suggested that CENP-B forms a more stable complex with the CENP-A nucleosome through specific interactions with CENP-A, if the CENP-B box is located proximal to the CENP-A nucleosome. Our in vivo assay also revealed that CENP-B binding in the vicinity of the CENP-A nucleosome substantially stabilizes the CENP-A nucleosome on alphoid DNA in human cells.
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