ArticleNucleic acids research2015
Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Upgraded circular single-stranded DNA regulators for multiple-input multiple-output gene circuits in mammalian cells.Science advances · 2025Article
- T7 phage-assisted evolution of riboswitches using error-prone replication and dual selection.Scientific reports · 2024Article
- Customizing cellular signal processing by synthetic multi-level regulatory circuits.Nature communications · 2023Review
- Model-Based Investigation of the Relationship between Regulation Level and Pulse Property of I1-FFL Gene Circuits.ACS synthetic biology · 2022Article
- Engineering a Circular Riboregulator inBiodesign research · 2020Article
- Novel bioactive natural products from bacteria via bioprospecting, genome mining and metabolic engineering.Microbial biotechnology · 2019Review
- Riboswitch identification using Ligase-Assisted Selection for the Enrichment of Responsive Ribozymes (LigASERR).Synthetic biology (Oxford, England) · 2019Article
- RNA-based dynamic genetic controllers: development strategies and applications.Current opinion in biotechnology · 2018Review
- Binary addition in a living cell based on riboregulation.PLoS genetics · 2018Article
- Model-based design of RNA hybridization networks implemented in living cells.Nucleic acids research · 2017Article
- Synthetic Gene Expression Circuits for Designing Precision Tools in Oncology.Frontiers in cell and developmental biology · 2017Review
- Cellular Delivery of RNA Nanoparticles.ACS combinatorial science · 2016Review
- Dual control system - A novel scaffolding architecture of an inducible regulatory device for the precise regulation of gene expression.Metabolic engineering · 2016Article
- WISB: Warwick Integrative Synthetic Biology Centre.Biochemical Society transactions · 2016Article
- Automated design of hammerhead ribozymes and validation by targeting the PABPN1 gene transcript.Nucleic acids research · 2016Article
- RNA-based gene circuits for cell regulation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2016Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organisms have different circuitries that allow converting signal molecule levels to changes in gene expression. An important challenge in synthetic biology involves the de novo design of RNA modules enabling dynamic signal processing in live cells. This requires a scalable methodology for sensing, transmission, and actuation, which could be assembled into larger signaling networks. Here, we present a biochemical strategy to design RNA-mediated signal transduction cascades able to sense small molecules and small RNAs. We design switchable functional RNA domains by using strand-displacement techniques. We experimentally characterize the molecular mechanism underlying our synthetic RNA signaling cascades, show the ability to regulate gene expression with transduced RNA signals, and describe the signal processing response of our systems to periodic forcing in single live cells. The engineered systems integrate RNA-RNA interaction with available ribozyme and aptamer elements, providing new ways to engineer arbitrary complex gene circuits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.