ArticleJournal of preventive medicine and hygiene2014
Molecular identification of Pseudomonas aeruginosa recovered from cystic fibrosis patients.
Article in Journal of preventive medicine and hygiene, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Exploring the occurrence of Pseudomonas aeruginosa and comprehensive whole genome analysis of the bcsir_p4_s20 strain from municipal wastewater in Chattogram.World journal of microbiology & biotechnology · 2025Article
- Detection of the epidemic Pseudomonas aeruginosa AUST-03 (ST242) strain in people with cystic fibrosis in South Africa.Pediatric pulmonology · 2024Article
- Systematic Review and Meta-Analysis on the Prevalence and Antibiotic Susceptibility Pattern in Pseudomonas aeruginosa Isolated from Cystic Fibrosis Patients.The Eurasian journal of medicine · 2024Article
- Antimicrobial Resistance, Genetic Lineages, and Biofilm Formation inAntibiotics (Basel, Switzerland) · 2023Article
- Isolation of a bacteriophage targeting Pseudomonas aeruginosa and exhibits a promising in vivo efficacy.AMB Express · 2023Article
- Article
- Prevalence and Phenotypic and Genotypic Resistance Mechanisms of Multidrug-ResistantJournal of environmental and public health · 2021Article
- Association of biofilm formation with multi drug resistance in clinical isolates ofEXCLI journal · 2020Article
- Recent Advances in Molecular Diagnosis ofFrontiers in microbiology · 2018Article
- Fast and specific detection of Pseudomonas Aeruginosa from other pseudomonas species by PCR.Annals of burns and fire disasters · 2016Article
- PCR-based assay for the rapid and precise distinction of Pseudomonas aeruginosa from other Pseudomonas species recovered from burns patients.Journal of preventive medicine and hygiene · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivePrecise identification of various morphotypes of Pseduomonas aeruginosa which developed during cystic fibrosis (CF) is of prime importance. We aimed to identify the isolates of P. aeruginosa recovered from CF patients at the genus and species level through primers targeting oprI and oprL genes via PCR.
methodsSputum samples or throat swabs were taken from 100 CF patients and plated on cetrimide agar. All suspected colonies were primarily screened for P. aeruginosa by a combination of phenotypic tests. Molecular identification of colonies was performed using specific primers for oprI and oprL genes.
resultsBased on phenotypic tests, P. aeruginosa isolates were recovered from 40% of CF patients. Forty isolates yielded amplicon of oprI gene using genus-specific primers confirming the identity of fluorescent pseudomonads. However, 37 of 40 isolates yielded amplicon of oprL gene using species-specific primers, verifying the identity of P. aeruginosa.
conclusionThis study showed that the species-specific PCR targeting oprL gene can be used as accurate test for identification of highly adaptable P. aeruginosa in CF patients. This procedure may provide a simple and reliable method for identification of various morphotypes.
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