ArticleOncotarget2015
Development and characterisation of a 3D multi-cellular in vitro model of normal human breast: a tool for cancer initiation studies.
Article in Oncotarget, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Construction and application of mammary and breast cancer organoids.Cell insight · 2026Review
- The Role that Biobanks Can Play in Driving Animal-Free Biomedical Research.Expert reviews in molecular medicine · 2026Review
- BIOPRINTING OF MICRODISSECTED TUMOR "CUBOIDS" IN HYDROGELS.bioRxiv : the preprint server for biology · 2025Article
- Engineering Heterogeneous Tumor Models for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Review
- Microdissected "cuboids" for microfluidic drug testing of intact tissues.Lab on a chip · 2021Article
- Upregulation of HIF1-α via an NF-κB/COX2 pathway confers proliferative dominance of HER2-negative ductal carcinoma in situ cells in response to inflammatory stimuli.Neoplasia (New York, N.Y.) · 2020Article
- A microfluidic platform for functional testing of cancer drugs on intact tumor slices.Lab on a chip · 2020Article
- Modeling chemical effects on breast cancer: the importance of the microenvironment in vitro.Integrative biology : quantitative biosciences from nano to macro · 2020Review
- Thread as a Low-Cost Material for Microfluidic Assays on Intact Tumor Slices.Micromachines · 2019Article
- Open multi-culture platform for simple and flexible study of multi-cell type interactions.Lab on a chip · 2018Article
- Luminal MCF-12A & myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast.Future science OA · 2018Article
- [Establishment of a living biobank : Improved guidance of precision cancer care with in vitro and in vivo cancer models].Der Pathologe · 2017Review
- Advances in the development of improved animal-free models for use in breast cancer biomedical research.Biophysical reviews · 2017Review
- PersonalizedCancer discovery · 2017Article
- A 3D in vitro model of the human breast duct: a method to unravel myoepithelial-luminal interactions in the progression of breast cancer.Breast cancer research : BCR · 2017Article
- Establishment of a normal-derived estrogen receptor-positive cell line comparable to the prevailing human breast cancer subtype.Oncotarget · 2017Article
- Correlation of SASH1 expression and ultrasonographic features in breast cancer.OncoTargets and therapy · 2017Article
- Integrins as architects of cell behavior.Molecular biology of the cell · 2016Review
- WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition.Journal of experimental & clinical cancer research : CR · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multicellular 3-dimensional (3D) in vitro models of normal human breast tissue to study cancer initiation are required. We present a model incorporating three of the major functional cell types of breast, detail the phenotype and document our breast cancer initiation studies. Myoepithelial cells and fibroblasts were isolated and immortalised from breast reduction mammoplasty samples. Tri-cultures containing non-tumorigenic luminal epithelial cells HB2, or HB2 overexpressing different HER proteins, together with myoepithelial cells and fibroblasts were established in collagen I. Phenotype was assessed morphologically and immunohistochemically and compared to normal breast tissue. When all three cell types were present, polarised epithelial structures with lumens and basement membrane production were observed, akin to normal human breast tissue. Overexpression of HER2 or HER2/3 caused a significant increase in size, while HER2 overexpression resulted in development of a DCIS-like phenotype. In summary, we have developed a 3D tri-cellular model of normal human breast, amenable to comparative analysis after genetic manipulation and with potential to dissect the mechanisms behind the early stages of breast cancer initiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.