ArticlePloS one2015
Tracking the Elusive Function of Bacillus subtilis Hfq.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 53 citations in OpenAlex.
- The sRNA LhrC5 and Hfq post-transcriptionally regulate ferritin expression inFrontiers in microbiology · 2026Article
- Remodeling of RNA-binding proteome and RNA-mediated regulation as a new layer of control of sporulation.mSystems · 2025Article
- A phosphate starvation induced small RNA promotes Bacillus biofilm formation.NPJ biofilms and microbiomes · 2024Article
- Staphylococcal aconitase expression during iron deficiency is controlled by an sRNA-driven feedforward loop and moonlighting activity.Nucleic acids research · 2024Article
- The previously uncharacterized RnpM (YlxR) protein modulates the activity of ribonuclease P in Bacillus subtilis in vitro.Nucleic acids research · 2024Article
- Article
- Small proteins in Gram-positive bacteria.FEMS microbiology reviews · 2023Article
- sRNA-controlled iron sparing response in Staphylococci.Nucleic acids research · 2022Article
- Role of Hfq in glucose utilization, biofilm formation and quorum sensing system in Bacillus subtilis.Biotechnology letters · 2022Article
- Comparative Transcriptomic Analysis ofFoods (Basel, Switzerland) · 2022Article
- Discovery and initial characterization of YloC, a novel endoribonuclease inRNA (New York, N.Y.) · 2022Article
- The RNA Chaperone Protein Hfq Regulates the Characteristic Sporulation and Insecticidal Activity ofFrontiers in microbiology · 2022Article
- Identification of RNAs bound by Hfq reveals widespread RNA partners and a sporulation regulator in the human pathogenRNA biology · 2021Article
- An RNA-centric global view ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Identification of an RNA sponge that controls the RoxS riboregulator of central metabolism in Bacillus subtilis.Nucleic acids research · 2021Article
- Non-B DNA-Forming Motifs Promote Mfd-Dependent Stationary-Phase Mutagenesis inMicroorganisms · 2021Article
- Diversity and Versatility in Small RNA-Mediated Regulation in Bacterial Pathogens.Frontiers in microbiology · 2021Review
- Grad-seq identifies KhpB as a global RNA-binding protein inmicroLife · 2021Article
- Staphylococcus aureus pigmentation is not controlled by Hfq.BMC research notes · 2020Article
- Regulation of mRNA Stability During Bacterial Stress Responses.Frontiers in microbiology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-binding protein Hfq is a key component of the adaptive responses of many proteobacterial species including Escherichia coli, Salmonella enterica and Vibrio cholera. In these organisms, the importance of Hfq largely stems from its participation to regulatory mechanisms involving small non-coding RNAs. In contrast, the function of Hfq in Gram-positive bacteria has remained elusive and somewhat controversial. In the present study, we have further addressed this point by comparing growth phenotypes and transcription profiles between wild-type and an hfq deletion mutant of the model Gram-positive bacterium, Bacillus subtilis. The absence of Hfq had no significant consequences on growth rates under nearly two thousand metabolic conditions and chemical treatments. The only phenotypic difference was a survival defect of B. subtilis hfq mutant in rich medium in stationary phase. Transcriptomic analysis correlated this phenotype with a change in the levels of nearly one hundred transcripts. Albeit a significant fraction of these RNAs (36%) encoded sporulation-related functions, analyses in a strain unable to sporulate ruled out sporulation per se as the basis of the hfq mutant's stationary phase fitness defect. When expressed in Salmonella, B. subtilis hfq complemented the sharp loss of viability of a degP hfq double mutant, attenuating the chronic σE-activated phenotype of this strain. However, B. subtilis hfq did not complement other regulatory deficiencies resulting from loss of Hfq-dependent small RNA activity in Salmonella indicating a limited functional overlap between Salmonella and B. subtilis Hfqs. Overall, this study confirmed that, despite structural similarities with other Hfq proteins, B. subtilis Hfq does not play a central role in post-transcriptional regulation but might have a more specialized function connected with stationary phase physiology. This would account for the high degree of conservation of Hfq proteins in all 17 B. subtilis strains whose genomes have been sequenced.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.