ArticleNature methods2015
Multispectral labeling technique to map many neighboring axonal projections in the same tissue.
Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Connectomic evidence that ordered activity drives neuromuscular network formation.Nature neuroscience · 2026Article
- Protein nanopore reveals the renin-angiotensin system crosstalk with single-amino-acid resolution.Nature chemistry · 2023Article
- Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin.Nature neuroscience · 2022Article
- Multicolor labeling of airway neurons and analysis of parasympathetic heterogeneity.Scientific reports · 2022Article
- Article
- Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain.Cells · 2020Article
- Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy.Nature communications · 2019Article
- Multispectral Live-Cell Imaging.Current protocols in cell biology · 2018Article
- Inhibitory circuit gating of auditory critical-period plasticity.Nature neuroscience · 2018Article
- Dopaminergic dysfunction in neurodevelopmental disorders: recent advances and synergistic technologies to aid basic research.Current opinion in neurobiology · 2018Review
- Applying systems-level spectral imaging and analysis to reveal the organelle interactome.Nature · 2017Article
- Article
- Article
- Extracting structural and functional features of widely distributed biological circuits with single cell resolution via tissue clearing and delivery vectors.Current opinion in biotechnology · 2016Review
- Detection of aberrant hippocampal mossy fiber connections: Ex vivo mesoscale diffusion MRI and microtractography with histological validation in a patient with uncontrolled temporal lobe epilepsy.Human brain mapping · 2016Article
- Article
- Neuroanatomical Tracing Techniques in the Ear: History, State of the Art, and Future Developments.Methods in molecular biology (Clifton, N.J.) · 2016Review
- Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping.Nature protocols · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We describe a method to map the location of axonal arbors of many individual neurons simultaneously via the spectral properties of retrogradely transported dye-labeled vesicles. We inject overlapping regions of an axon target area with three or more different colored retrograde tracers. On the basis of the combinations and intensities of the colors in the individual vesicles transported to neuronal somata, we calculate the projection sites of each neuron's axon. This neuronal positioning system (NPS) enables mapping of many axons in a simple automated way. In our experiments, NPS combined with spectral (Brainbow) labeling of the input to autonomic ganglion cells showed that the locations of ganglion cell projections to a mouse salivary gland related to the identities of their preganglionic axonal innervation. NPS could also delineate projections of many axons simultaneously in the mouse central nervous system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.