Evidence map›Paper›PMID 25915034›Full record

ArticleNature materials2015

In vivo delivery of transcription factors with multifunctional oligonucleotides.

Kunwoo Lee, Mohammad Rafi, Xiaojian Wang, Kiana Aran, Xuli Feng, Carlo Lo Sterzo, Richard Tang, Nithya Lingampalli, Hyun Jin Kim, Niren Murthy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature materials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
  2. Article
  3. Bioactive materials forBioactive materials · 2024
    Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Near-infrared optogenetic engineering of photothermal nanoCRISPR for programmable genome editing.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  11. Article
  12. Article
  13. Recent developments in intracellular protein delivery.Current opinion in biotechnology · 2018
    Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Nanobubble Contrast Enhanced Ultrasound Imaging: A Review.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kunwoo Lee1] Department of Bioengineering, University of California, Hearst Memorial Mining Building, Room 284 Berkeley, California 94720, USA [2] UC Berkeley and UCSF Joint Graduate Program in Bioengineering, Berkeley/San Francisco, USA.
Mohammad RafiDepartment of Bioengineering, University of California, Hearst Memorial Mining Building, Room 284 Berkeley, California 94720, USA.
Xiaojian WangDepartment of Bioengineering, University of California, Hearst Memorial Mining Building, Room 284 Berkeley, California 94720, USA.
Kiana AranDepartment of Bioengineering, University of California, Hearst Memorial Mining Building, Room 284 Berkeley, California 94720, USA.
Xuli FengDepartment of Bioengineering, University of California, Hearst Memorial Mining Building, Room 284 Berkeley, California 94720, USA.
Carlo Lo SterzoDepartment of Bioengineering, University of California, Hearst Memorial Mining Building, Room 284 Berkeley, California 94720, USA.
Richard TangDepartment of Bioengineering, University of California, Hearst Memorial Mining Building, Room 284 Berkeley, California 94720, USA.
Nithya LingampalliDepartment of Bioengineering, University of California, Hearst Memorial Mining Building, Room 284 Berkeley, California 94720, USA.
Hyun Jin KimDepartment of Bioengineering, University of California, Hearst Memorial Mining Building, Room 284 Berkeley, California 94720, USA.
Niren Murthy1] Department of Bioengineering, University of California, Hearst Memorial Mining Building, Room 284 Berkeley, California 94720, USA [2] UC Berkeley and UCSF Joint Graduate Program in Bioengineering, Berkeley/San Francisco, USA.

Funding

Spatiotemporal control of reactive oxygen species in T cellsR01AI088023 · NIAID · GEORGIA INSTITUTE OF TECHNOLOGY · PI KEMP, MELISSA LAMBETH · 2011 to 2014
$1.4M
Rapid antimicrobial susceptibility determination of bacterial pathogensR01AI107116 · NIAID · GEORGIA INSTITUTE OF TECHNOLOGY · PI DICKSON, ROBERT M, TZENG, YIH-LING · 2014 to 2017
$1.4M
Maltodextrin based antibioticsR21AI098799 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI MURTHY, NIREN · 2012 to 2013
$436k
NHLBI NIH HHS HHSN268201000043CNIAID NIH HHS R01 AI088023NIAID NIH HHS R01 AI088023-03NIAID NIH HHS R01 AI107116NIAID NIH HHS R01 AI107116-01NIAID NIH HHS R21 AI098799PHS HHS U01 268201000043C-0-0-1
6 · The paper itself

Abstract

Therapeutics based on transcription factors have the potential to revolutionize medicine but have had limited clinical success as a consequence of delivery problems. The delivery of transcription factors is challenging because it requires the development of a delivery vehicle that can complex transcription factors, target cells and stimulate endosomal disruption, with minimal toxicity. Here, we present a multifunctional oligonucleotide, termed DARTs (DNA assembled recombinant transcription factors), which can deliver transcription factors with high efficiency in vivo. DARTs are composed of an oligonucleotide that contains a transcription-factor-binding sequence and hydrophobic membrane-disruptive chains that are masked by acid-cleavable galactose residues. DARTs have a unique molecular architecture, which allows them to bind transcription factors, trigger endocytosis in hepatocytes, and stimulate endosomal disruption. The DARTs have enhanced uptake in hepatocytes as a result of their galactose residues and can disrupt endosomes efficiently with minimal toxicity, because unmasking of their hydrophobic domains selectively occurs in the acidic environment of the endosome. We show that DARTs can deliver the transcription factor nuclear erythroid 2-related factor 2 (Nrf2) to the liver, catalyse the transcription of Nrf2 downstream genes, and rescue mice from acetaminophen-induced liver injury.

Indexed as

Alanine TransaminaseAnimalsDNADrug Delivery SystemsEndosomesHepatocytesHep G2 CellsHumansHydrogen-Ion ConcentrationHydrolysisLiverMiceNF-E2-Related Factor 2OligonucleotidesOxidative StressSpectrometry, FluorescenceAlanine TransaminaseDNANfe2l2 protein, mouseNF-E2-Related Factor 2OligonucleotidesTranscription Factors

Identifiers

PMID25915034
PMCPMC4617181

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.