ArticleNature2015
Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles.
Article in Nature, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 82 citations in OpenAlex.
- Actin network evolution as a key driver of eukaryotic diversification.Journal of cell science · 2024Review
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Single particle cryo-EM analysis of Rickettsia conorii Sca2 reveals a formin-like core.Journal of structural biology · 2023Article
- Diverse cytomotive actins and tubulins share a polymerization switch mechanism conferring robust dynamics.Science advances · 2023Article
- Structural mechanism for bidirectional actin cross-linking by T-plastin.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Article
- Unique Properties of the Alpha-Helical DNA-Binding Protein KfrA Encoded by the IncU Incompatibility Group Plasmid RA3 and Its Host-Dependent Role in Plasmid Maintenance.Applied and environmental microbiology · 2021Article
- The C-terminal region of the plasmid partitioning protein TubY is a tetramer that can bind membranes and DNA.The Journal of biological chemistry · 2020Article
- Nucleoid-mediated positioning and transport in bacteria.Current genetics · 2020Review
- Polymerization in the actin ATPase clan regulates hexokinase activity in yeast.Science (New York, N.Y.) · 2020Article
- Identification and characterization of novel filament-forming proteins in cyanobacteria.Scientific reports · 2020Article
- The structure of a 15-stranded actin-like filament from Clostridium botulinum.Nature communications · 2019Article
- Structures of maintenance of carboxysome distribution Walker-box McdA and McdB adaptor homologs.Nucleic acids research · 2019Article
- Plasmid Localization and Partition inEcoSal Plus · 2019Review
- Conservation of conformational dynamics across prokaryotic actins.PLoS computational biology · 2019Article
- Locating macromolecules and determining structures inside bacterial cells using electron cryotomography.Biochimica et biophysica acta. Proteins and proteomics · 2018Review
- Atomic insights into the genesis of cellular filaments by globular proteins.Nature structural & molecular biology · 2018Article
- Spread and Persistence of Virulence and Antibiotic Resistance Genes: A Ride on the F Plasmid Conjugation Module.EcoSal Plus · 2018Review
- Structural transitions of F-actin upon ATP hydrolysis at near-atomic resolution revealed by cryo-EM.Nature structural & molecular biology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Active segregation of Escherichia coli low-copy-number plasmid R1 involves formation of a bipolar spindle made of left-handed double-helical actin-like ParM filaments. ParR links the filaments with centromeric parC plasmid DNA, while facilitating the addition of subunits to ParM filaments. Growing ParMRC spindles push sister plasmids to the cell poles. Here, using modern electron cryomicroscopy methods, we investigate the structures and arrangements of ParM filaments in vitro and in cells, revealing at near-atomic resolution how subunits and filaments come together to produce the simplest known mitotic machinery. To understand the mechanism of dynamic instability, we determine structures of ParM filaments in different nucleotide states. The structure of filaments bound to the ATP analogue AMPPNP is determined at 4.3 Å resolution and refined. The ParM filament structure shows strong longitudinal interfaces and weaker lateral interactions. Also using electron cryomicroscopy, we reconstruct ParM doublets forming antiparallel spindles. Finally, with whole-cell electron cryotomography, we show that doublets are abundant in bacterial cells containing low-copy-number plasmids with the ParMRC locus, leading to an asynchronous model of R1 plasmid segregation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.