ArticleFrontiers in microbiology2015
Engineering mediator-based electroactivity in the obligate aerobic bacterium Pseudomonas putida KT2440.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 120 citations in OpenAlex.
- Enhancement Strategies for Bioelectrocatalytic Conversion of Organic Waste.Chem & bio engineering · 2026Review
- Genetically Reprogramming Crops and Rhizobacteria for Nutritional Iron Biofortification.ACS synthetic biology · 2025Review
- Synthetic Whole-Cell Bioelectronic Chemical Sensing withChem & bio engineering · 2025Article
- The TonB-Dependent Transport System Facilitates the Uptake of Inorganic Metal Mediators in Pseudomonas putida KT2440 in a Bioelectrochemical System.Microbial biotechnology · 2025Article
- Biochemical production with microbial bioelectrochemical systems.Current opinion in biotechnology · 2025Review
- Systems biology of electrogenic Pseudomonas putida - multi-omics insights and metabolic engineering for enhanced 2-ketogluconate production.Microbial cell factories · 2024Article
- Microbial electrosynthesis: opportunities for microbial pure cultures.Trends in biotechnology · 2024Review
- Establishing a straightforward I-SceI-mediated recombination one-plasmid system for efficient genome editing in P. putida KT2440.Microbial biotechnology · 2024Article
- Electricity generation byEngineering microbiology · 2024Article
- Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose.Nature communications · 2024Article
- Direct ammonium oxidation to nitrogen gas (Dirammox) inEnvironmental science and ecotechnology · 2023Article
- Exploiting synergies between microbial electrochemical technologies and synthetic biology.Microbial biotechnology · 2023Article
- Screening of natural phenazine producers for electroactivity in bioelectrochemical systems.Microbial biotechnology · 2023Article
- The two faces of pyocyanin - why and how to steer its production?World journal of microbiology & biotechnology · 2023Review
- Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing.Applied and environmental microbiology · 2022Article
- Editorial: Electrobiotechnology Towards Sustainable Bioeconomy: Fundamental, Optimization and Applications.Frontiers in bioengineering and biotechnology · 2022Article
- Bridging the gap between microbial limits and extremes in space: space microbial biotechnology in the next 15 years.Microbial biotechnology · 2022Article
- Using structure-function relationships to understand the mechanism of phenazine-mediated extracellular electron transfer iniScience · 2021Article
- The anoxic electrode-driven fructose catabolism of Pseudomonas putida KT2440.Microbial biotechnology · 2021Article
- Role of phenazine-enzyme physiology for current generation in a bioelectrochemical system.Microbial biotechnology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas putida strains are being developed as microbial production hosts for production of a range of amphiphilic and hydrophobic biochemicals. P. putida's obligate aerobic growth thereby can be an economical and technical challenge because it requires constant rigorous aeration and often causes reactor foaming. Here, we engineered a strain of P. putida KT2440 that can produce phenazine redox-mediators from Pseudomonas aeruginosa to allow partial redox balancing with an electrode under oxygen-limited conditions. P. aeruginosa is known to employ its phenazine-type redox mediators for electron exchange with an anode in bioelectrochemical systems (BES). We transferred the seven core phenazine biosynthesis genes phzA-G and the two specific genes phzM and phzS required for pyocyanin synthesis from P. aeruginosa on two inducible plasmids into P. putida KT2440. The best clone, P. putida pPhz, produced 45 mg/L pyocyanin over 25 h of growth, which was visible as blue color formation and is comparable to the pyocyanin production of P. aeruginosa. This new strain was then characterized under different oxygen-limited conditions with electrochemical redox control and changes in central energy metabolism were evaluated in comparison to the unmodified P. putida KT2440. In the new strain, phenazine synthesis with supernatant concentrations up to 33 μg/mL correlated linearly with the ability to discharge electrons to an anode, whereby phenazine-1-carboxylic acid served as the dominating redox mediator. P. putida pPhz sustained strongly oxygen-limited metabolism for up to 2 weeks at up to 12 μA/cm(2) anodic current density. Together, this work lays a foundation for future oxygen-limited biocatalysis with P. putida strains.
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