ArticleFrontiers in cellular neuroscience2015
Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes.
Article in Frontiers in cellular neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Chloride Homeostasis Failure in Human Disease: KCC2/NKCC1 Microdomain Dysfunction as a Driver of Cortical Network Collapse.International journal of molecular sciences · 2026Review
- Neuronal extracellular vesicles influence the expression, degradation and oligomeric state of fructose 1,6-bisphosphatase 2 in astrocytes affecting their glycolytic capacity.Scientific reports · 2024Article
- Astrocytic chloride is brain state dependent and modulates inhibitory neurotransmission in mice.Nature communications · 2023Article
- Cocaine experience induces functional adaptations in astrocytes: Implications for synaptic plasticity in the nucleus accumbens shell.Addiction biology · 2021Article
- Activation of Glutamate Transporter-1 (GLT-1) Confers Sex-Dependent Neuroprotection in Brain Ischemia.Brain sciences · 2021Article
- Valproic acid-exposed astrocytes impair inhibitory synapse formation and function.Scientific reports · 2021Article
- Brain Volume Loss, Astrocyte Reduction, and Inflammation in Anorexia Nervosa.Advances in neurobiology · 2021Article
- Antioxidants and Neuron-Astrocyte Interplay in Brain Physiology: Melatonin, a Neighbor to Rely on.Neurochemical research · 2021Review
- Negative Feedback Role of Astrocytes in Shaping Excitation in Brain Cell Co-cultures.Frontiers in cellular neuroscience · 2021Article
- Article
- Microglia: sculptors of neuropathic pain?Royal Society open science · 2020Review
- A thermodynamic function of glycogen in brain and muscle.Progress in neurobiology · 2020Article
- AMPK activation protects astrocytes from hypoxia‑induced cell death.International journal of molecular medicine · 2020Article
- The reduction of astrocytes and brain volume loss in anorexia nervosa-the impact of starvation and refeeding in a rodent model.Translational psychiatry · 2019Article
- Neuron-glia interactions in the pathophysiology of epilepsy.Nature reviews. Neuroscience · 2019Review
- MMP-1 overexpression selectively alters inhibition in D1 spiny projection neurons in the mouse nucleus accumbens core.Scientific reports · 2018Article
- Article
- Roles of Specialized Pro-Resolving Lipid Mediators in Cerebral Ischemia Reperfusion Injury.Frontiers in neurology · 2018Review
- The Role of Astrocytes in Neuroprotection after Brain Stroke: Potential in Cell Therapy.Frontiers in molecular neuroscience · 2017Review
- Roles of Glial Cells in Sculpting Inhibitory Synapses and Neural Circuits.Frontiers in molecular neuroscience · 2017Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gamma aminobutric acid (GABA) is the major inhibitory neurotransmitter in the adult brain and mechanisms of GABAergic inhibition have been intensely investigated in the past decades. Recent studies provided evidence for an important role of astrocytes in shaping GABAergic currents. One of the most obvious, but yet poorly understood, mechanisms of the cross-talk between GABAergic currents and astrocytes is metabolism including neurotransmitter homeostasis. In particular, how modulation of GABAergic currents by astrocytes depends on key enzymes involved in cellular metabolism remains largely unknown. To address this issue, we have considered two simple models of neuronal culture (NC): nominally astrocyte-free NC and neuronal-astrocytic co-cultures (ANCC). Miniature Inhibitory Postsynaptic Currents (mIPSCs) were recorded in control conditions and in the presence of different enzyme blockers. We report that enrichment of NC with astrocytes results in a marked increase in mIPSC frequency. This enhancement of GABAergic activity was accompanied by increased number of GAD65 and vGAT puncta, indicating that at least a part of the frequency enhancement was due to increased number of synaptic contacts. Inhibition of glutamine synthetase (Glns) (with MSO) strongly reduced mIPSC frequency in ANCC but had no effect in NC. Moreover, treatment of ANCC with inhibitor of glycogen phosphorylase (Gys) (BAYU6751) or with selective inhibitor of astrocytic Krebs cycle, fluoroacetate, resulted in a marked reduction of mIPSC frequency in ANCC having no effect in NC. We conclude that GABAergic synaptic transmission strongly depends on neuron-astrocyte interaction in a manner dependent on key metabolic enzymes as well as on the Krebs cycle.
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