ArticleBreeding science2014
A novel GmFAD3-2a mutant allele developed through TILLING reduces α-linolenic acid content in soybean seed oil.
Article in Breeding science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 40 citations in OpenAlex.
- The current state of Japanese soybean breeding using DNA marker technology.Breeding science · 2026Article
- Integrative Identification of Chloroplast Metabolism-Related RETICULATA-RELATED Genes in Soybean.Plants (Basel, Switzerland) · 2025Article
- Development and Validation of Kompetitive Allele-Specific Polymerase Chain Reaction Markers for Seed Protein Content in Soybean.Plants (Basel, Switzerland) · 2024Article
- Regulation of seed traits in soybean.aBIOTECH · 2023Review
- WGCNA and transcriptome profiling reveal hub genes for key development stage seed size/oil content between wild and cultivated soybean.BMC genomics · 2023Article
- Diversity of fatty acid biosynthesis genes across the soybean pangenome.The plant genome · 2023Article
- Genetic mapping and functional genomics of soybean seed protein.Molecular breeding : new strategies in plant improvement · 2023Article
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- Development of Ethyl Methanesulfonate Mutant Edamame Soybean (Plants (Basel, Switzerland) · 2022Article
- TILLING-by-SequencingInternational journal of molecular sciences · 2021Article
- Single-base deletion inBreeding science · 2020Article
- TILLING by Sequencing: A Successful Approach to Identify Rare Alleles in Soybean Populations.Genes · 2019Article
- Accelerating Soybean Breeding in a CO2-Supplemented Growth Chamber.Plant & cell physiology · 2019Article
- Improved oil quality in transgenic soybean seeds by RNAi-mediated knockdown of GmFAD2-1B.Transgenic research · 2018Article
- Genome-wide association studies dissect the genetic networks underlying agronomical traits in soybean.Genome biology · 2017Article
- Mutagenesis and TILLING to Dissect Gene Function in Plants.Current genomics · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soybean (Glycine max (L.) Merr.) oil typically contains 8% α-linolenic acid that is highly unstable and easily oxidized. This property is undesirable in many food and industrial applications. Genetic strategies for reducing α-linolenic acid content would enhance the commercial value. However, genetic resources for low α-linolenic acid content are limited among natural soybean variations. Microsomal omega-3-fatty acid desaturase (FAD3) is responsible for the synthesis of α-linolenic acid in the polyunsaturated fatty acid pathway. There are four FAD3 homologs (Glyma02g39230, Glyma11g27190, Glyma14g37350 and Glyma18g06950) in the soybean genome. While non-functional alleles have been reported for Glyma02g39230 (GmFAD3-1a) and Glyma14g37350 (GmFAD3-1b), little variation is seen in Glyma18g06950 (GmFAD3-2a). We isolated seven mutant GmFAD3-2a alleles, each containing a single-nucleotide substitution, from 39,100 independent mutant lines by using targeting induced local lesions in genomes (TILLING). Analysis of GmFAD3-2a transcripts and enzyme activities revealed that one missense mutant, 'P1-A9', contains a non-functional allele of GmFAD3-2a. By combining three non-functional alleles (GmFAD3-1a, GmFAD3-1b, and GmFAD3-2a), we generated soybean lines containing <2% α-linolenic acid in their seeds. The reverse-genetics-based development of novel mutant alleles in the fatty acid metabolic pathway will allow the improvement of soybean with better oil quality through conventional breeding.
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