Evidence map›Paper›PMID 25913508›Full record

ArticleScientific reports2015

Nucleotide sequence-homology-independent breakdown of transgenic resistance by more virulent virus strains and a potential solution.

Yi-Jung Kung, Bang-Jau You, Joseph A J Raja, Kuan-Chun Chen, Chiung-Huei Huang, Huey-Jiunn Bau, Ching-Fu Yang, Chung-Hao Huang, Chung-Ping Chang, Shyi-Dong Yeh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
4.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 27 citations in OpenAlex.

  1. RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025
    Review
  2. Article
  3. Article
  4. RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021
    Review
  5. Article
  6. Review
  7. Review
  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Yi-Jung Kung1] Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C. [2] NCHU-UCD Plant and Food Biotechnology Center, National Chung Hsing University, Taiwan, R.O.C.
Bang-Jau You1] Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C. [2] Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan, R.O.C.
Joseph A J Raja1] Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C. [2] NCHU-UCD Plant and Food Biotechnology Center, National Chung Hsing University, Taiwan, R.O.C.
Kuan-Chun ChenDepartment of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Chiung-Huei HuangDepartment of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Huey-Jiunn BauDepartment of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Ching-Fu Yang1] Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C. [2] Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Chung-Hao HuangDepartment of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Chung-Ping ChangDepartment of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Shyi-Dong Yeh1] Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C. [2] NCHU-UCD Plant and Food Biotechnology Center, National Chung Hsing University, Taiwan, R.O.C. [3] Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan, R.O.C.
National Chung Hsing University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Controlling plant viruses by genetic engineering, including the globally important Papaya ringspot virus (PRSV), mainly involves coat protein (CP) gene mediated resistance via post-transcriptional gene silencing (PTGS). However, the breakdown of single- or double-virus resistance in CP-gene-transgenic papaya by more virulent PRSV strains has been noted in repeated field trials. Recombination analysis revealed that the gene silencing suppressor HC-Pro or CP of the virulent PRSV strain 5-19 is responsible for overcoming CP-transgenic resistance in a sequence-homology-independent manner. Transient expression assays using agro-infiltration in Nicotiana benthamiana plants indicated that 5-19 HC-Pro exhibits stronger PTGS suppression than the transgene donor strain. To disarm the suppressor from the virulent strain, transgenic papaya lines were generated carrying untranslatable 5-19 HC-Pro, which conferred complete resistance to 5-19 and other geographic PRSV strains. Our study suggested the potential risk of the emergence of more virulent virus strains, spurred by the deployment of CP-gene-transgenic crops, and provides a strategy to combat such strains.

Indexed as

Capsid ProteinsCaricaPlant DiseasesPlants, Genetically ModifiedPlant VirusesSequence Homology, Nucleic AcidTransgenesVirulenceCapsid Proteins

Identifiers

PMID25913508
PMCPMC5386206
OpenAlexW1971963860

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.