ArticlePlant biotechnology journal2016
Synthesis of oleyl oleate wax esters in Arabidopsis thaliana and Camelina sativa seed oil.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
- Metabolic engineering of cereal lipids: from omega-3 fatty acids to wax esters and pheromones.Advanced biotechnology · 2026Review
- Reducing the Sinapine Levels of Camelina sativa Seeds Through Targeted Genome Editing of REF1.Plant biotechnology journal · 2026Article
- Overexpression of jojoba wax ester synthase in poplar increases foliar lipid accumulation, alters stomatal conductance, and increases water use efficiency.Plant biology (Stuttgart, Germany) · 2025Article
- The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets.The Plant cell · 2025Article
- Ectopic expression of wax ester synthase under a wood-specific promoter enhances cell wall production and wood hydrophobicity.Biotechnology for biofuels and bioproducts · 2025Article
- Born of frustration: the emergence of Camelina sativa as a platform for lipid biotechnology.Plant physiology · 2025Review
- Exploring genetic diversity, population structure, and subgenome differences in the allopolyploidHorticulture research · 2024Article
- The production of wax esters in transgenic plants: towards a sustainable source of bio-lubricants.Journal of experimental botany · 2022Review
- Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina.Biotechnology for biofuels · 2021Article
- Lipases of germinating jojoba seeds efficiently hydrolyze triacylglycerols and wax esters and display wax ester-synthesizing activity.BMC plant biology · 2021Article
- Interactions between genetics and environment shape Camelina seed oil composition.BMC plant biology · 2020Article
- The genome of jojoba (Science advances · 2020Article
- Metabolic Engineering a Model OilseedFrontiers in plant science · 2020Review
- Origin and Evolution of Diploid and AllopolyploidThe Plant cell · 2019Article
- Camelina sativa, an oilseed at the nexus between model system and commercial crop.Plant cell reports · 2018Review
- High-level accumulation of oleyl oleate in plant seed oil by abundant supply of oleic acid substrates to efficient wax ester synthesis enzymes.Biotechnology for biofuels · 2018Article
- Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenicBiotechnology for biofuels · 2018Article
- Tailoring the composition of novel wax esters in the seeds of transgenic Camelina sativa through systematic metabolic engineering.Plant biotechnology journal · 2017Article
- Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil.Plant physiology · 2017Article
- Two bifunctional enzymes from the marine protistBiotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Seed oil composed of wax esters with long-chain monoenoic acyl moieties represents a high-value commodity for industry. Such plant-derived sperm oil-like liquid wax esters are biodegradable and can have excellent properties for lubrication. In addition, wax ester oil may represent a superior substrate for biodiesel production. In this study, we demonstrate that the low-input oil seed crop Camelina sativa can serve as a biotechnological platform for environmentally benign wax ester production. Two biosynthetic steps catalysed by a fatty alcohol-forming acyl-CoA reductase (FAR) and a wax ester synthase (WS) are sufficient to achieve wax ester accumulation from acyl-CoA substrates. To produce plant-derived sperm oil-like liquid wax esters, the WS from Mus musculus (MmWS) or Simmondsia chinensis (ScWS) were expressed in combination with the FAR from Mus musculus (MmFAR1) or Marinobacter aquaeolei (MaFAR) in seeds of Arabidopsis thaliana and Camelina sativa. The three analysed enzyme combinations Oleo3:mCherry:MmFAR1∆c/Oleo3:EYFP:MmWS, Oleo3:mCherry:MmFAR1∆c/ScWS and MaFAR/ScWS showed differences in the wax ester molecular species profiles and overall biosynthetic performance. By expressing MaFAR/ScWS in Arabidopsis or Camelina up to 59% or 21% of the seed oil TAGs were replaced by wax esters, respectively. This combination also yielded wax ester molecular species with highest content of monounsaturated acyl moieties. Expression of the enzyme combinations in the Arabidopsis fae1 fad2 mutant background high in oleic acid resulted in wax ester accumulation enriched in oleyl oleate (18:1/18:1 > 60%), suggesting that similar values may be obtained with a Camelina high oleic acid line.
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Registered trials
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