ArticleTransfusion2015
Effects of nitric oxide and its congeners on sickle red blood cell deformability.
Article in Transfusion, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Red blood cell mechanical sensitivity improves in patients with sickle cell disease undergoing chronic transfusion after prolonged, subhemolytic shear exposure.Transfusion · 2018Trial
- Complete Blood Count Profile in Steady State Sickle Cell Disease Patient: A Systematic Review and Meta-Analysis.Health science reports · 2026Article
- Evaluation of the effects of rosmarinic acid on red blood cell deformability, morphology, and nitric oxide in rat lower limb skeletal muscle ischemia-reperfusion injury.Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES · 2025Article
- Article
- Cellular microdomains for nitric oxide signaling in endothelium and red blood cells.Nitric oxide : biology and chemistry · 2020Review
- Metabolic Reprogramming of Mouse Bone Marrow Derived Macrophages Following Erythrophagocytosis.Frontiers in physiology · 2020Article
- Development of Zinc Chelating Resin Polymer Beads for the Removal of Cell-Free Hemoglobin.Annals of biomedical engineering · 2019Article
- Effects of Polyphenol Compounds and Nitrogen Oxide Donors on Lipid Oxidation, Membrane-Skeletal Proteins, and Erythrocyte Structure under Hypoxia.BioMed research international · 2019Article
- Nitrosyl-hemoglobin formation in rodent and human venous erythrocytes reflects NO formation from the vasculature in vivo.PloS one · 2018Article
- Erythrocytes and Vascular Function: Oxygen and Nitric Oxide.Frontiers in physiology · 2018Review
- Nitric oxide pathology and therapeutics in sickle cell disease.Clinical hemorheology and microcirculation · 2018Article
- On the Effects of Reactive Oxygen Species and Nitric Oxide on Red Blood Cell Deformability.Frontiers in physiology · 2018Article
- Structural and Functional Insight of Sphingosine 1-Phosphate-Mediated Pathogenic Metabolic Reprogramming in Sickle Cell Disease.Scientific reports · 2017Article
- Potential therapeutic action of nitrite in sickle cell disease.Redox biology · 2017Article
- Modulation of Local and Systemic Heterocellular Communication by Mechanical Forces: A Role of Endothelial Nitric Oxide Synthase.Antioxidants & redox signaling · 2017Review
- Red Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia.Antioxidants & redox signaling · 2017Review
- Comparative study of sickle cell anemia and hemoglobin SC disease: clinical characterization, laboratory biomarkers and genetic profiles.BMC hematology · 2017Article
- The role of blood rheology in sickle cell disease.Blood reviews · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSickle cell disease (SCD) is characterized by hemoglobin polymerization upon deoxygenation. Polymerization causes the sickle cells to become rigid and misshapen (sickling). Red blood cell (RBC) dehydration greatly increases polymerization. Cycles of sickling and unsickling cause an influx of calcium that leads to loss of potassium via the calcium-activated Gardos channel, which dehydrates the cells leading to increased polymerization. In this study the effects of nitric oxide (NO) and its congeners on RBC deformability were examined, focusing on sickle RBCs (sRBCs). STUDY DESIGN AND
methodsRBCs from patients with SCD and from nonpatients were exposed to various compounds that release NO or its congeners. Intracellular calcium was increased using a calcium ionophore or cycling of oxygen tension for sRBCs. Deformability was measured by laser-assisted osmotic gradient ektacytometry.
resultsConsistent with a previous report, sodium nitroprusside (SNP) was found to protect against calcium-induced loss of deformability in normal RBCs, but (contrary to some previous reports) no effect of any NO donors was observed when calcium influx was not induced. Importantly, in studies of deoxygenation-induced dehydration of sRBCs, SNP resulted in substantial improvements in deformability (p = 0.036) and hydration (p = 0.024). Sodium nitrite showed similar trends. SNP was shown to have no effect on calcium influx, but reduced potassium efflux.
conclusionThese data suggest that SNP and perhaps certain nitrogen oxides (like nitrite) inhibit the Gardos channel and may be able to protect sickle cells from dehydration and thereby improve outcome in the disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.