ArticleThe Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases
Detection of E. coli O157:H7 and Shigella dysenteriae toxins in clinical samples by PCR-ELISA.
Article in The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 52 citations in OpenAlex.
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- Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review.Heliyon · 2023Article
- Gut-Thyroid axis: How gut microbial dysbiosis associated with euthyroid thyroid cancer.Journal of Cancer · 2022Article
- Gut microbial dysbiosis and its association with esophageal cancer.Journal of applied biomedicine · 2021Article
- Occurrence of Virulence Genes and Antimicrobial Resistance ofVeterinary medicine international · 2021Article
- Identification of Dysfunctional Gut Microbiota Through Rectal Swab in Patients with Different Severity of Acute Pancreatitis.Digestive diseases and sciences · 2020Article
- Differentiation ofFood science & nutrition · 2020Article
- Article
- Molecular characterization of alterations in the intestinal microbiota of patients with grade 3 hypertension.International journal of molecular medicine · 2019Article
- Three Dimensional Vero Cell-Platform for Rapid and Sensitive Screening of Shiga-Toxin ProducingFrontiers in microbiology · 2019Article
- Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay.Sensors (Basel, Switzerland) · 2017Article
- Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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4 authors at 2 institutions in 1 country.
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Abstract
Shiga toxin producing bacteria are potential causes of serious human disease such as hemorrhagic colitis, severe inflammations of ileocolonic regions of gastrointestinal tract, thrombocytopenia, septicemia, malignant disorders in urinary ducts, hemolytic uremic syndrome (HUS). Shiga toxin 1 (stx1), shiga toxin 2 (stx2), or a combination of both are responsible for most clinical symptoms of these diseases. A lot of methods have been developed so far to detect shiga toxins such as cell culture, ELISA, and RFPLA, but due to high costs and labor time in addition to low sensitivity, they have not received much attention. In this study, PCR-ELISA method was used to detect genes encoding shiga toxins1 and 2 (stx1 and stx2). To detect stx1 and stx2 genes, two primer pairs were designed for Multiplex-PCR then PCR-ELISA. PCR products (490 and 275, respectively) were subsequently verified by sequencing. Sensitivity and specificity of PCR-ELISA method were determined by using genome serial dilution and Enterobacteria strains. PCR-ELISA method used in this study proved to be a rapid and precise approach to detect different types of shiga toxins and can be used to detect bacterial genes encoding shiga toxins.
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