Evidence map›Paper›PMID 25909817›Full record

ArticleScientific reports2015

MicroRNA transcriptomes of distinct human NK cell populations identify miR-362-5p as an essential regulator of NK cell function.

Fang Ni, Chuang Guo, Rui Sun, Binqing Fu, Yue Yang, Lele Wu, Sitong Ren, Zhigang Tian, Haiming Wei

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 66 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Killing the killers: Natural killer cell therapy targeting glioma stem cells in high-grade glioma.Molecular therapy : the journal of the American Society of Gene Therapy · 2025
    Review
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Article
  14. Review
  15. Review
  16. Article
  17. Review
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Fang Ni1] Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Science and Medical Center, University of Science and Technology of China, 443 Huang-Shan Road, Hefei 230027, China [2] Department of Pathophysiology, Anhui Medical University, 81 Mei-Shan Road, Hefei, Anhui Province 230032, China.
Chuang GuoInstitute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Science and Medical Center, University of Science and Technology of China, 443 Huang-Shan Road, Hefei 230027, China.
Rui SunInstitute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Science and Medical Center, University of Science and Technology of China, 443 Huang-Shan Road, Hefei 230027, China.
Binqing Fu1] Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Science and Medical Center, University of Science and Technology of China, 443 Huang-Shan Road, Hefei 230027, China [2] Hefei National Laboratory for Physical Sciences at Microscale, 96 Jin-Zhai Road, Hefei, Anhui Province 230026, China.
Yue YangDepartment of Pathophysiology, Anhui Medical University, 81 Mei-Shan Road, Hefei, Anhui Province 230032, China.
Lele WuDepartment of Pathophysiology, Anhui Medical University, 81 Mei-Shan Road, Hefei, Anhui Province 230032, China.
Sitong RenDepartment of Pathophysiology, Anhui Medical University, 81 Mei-Shan Road, Hefei, Anhui Province 230032, China.
Zhigang Tian1] Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Science and Medical Center, University of Science and Technology of China, 443 Huang-Shan Road, Hefei 230027, China [2] Hefei National Laboratory for Physical Sciences at Microscale, 96 Jin-Zhai Road, Hefei, Anhui Province 230026, China.
Haiming WeiInstitute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Science and Medical Center, University of Science and Technology of China, 443 Huang-Shan Road, Hefei 230027, China.
University of Science and Technology of China · CNAnhui Medical University · CNHefei National Center for Physical Sciences at Nanoscale · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Natural killer (NK) cells are critical effectors in the immune response against malignancy and infection, and microRNAs (miRNAs) play important roles in NK cell biology. Here we examined miRNA profiles of human NK cells from different cell compartments (peripheral blood, cord blood, and uterine deciduas) and of NKT and T cells from peripheral blood, and we identified a novel miRNA, miR-362-5p, that is highly expressed in human peripheral blood NK (pNK) cells. We also demonstrated that CYLD, a negative regulator of NF-κB signaling, was a target of miR-362-5p in NK cells. Furthermore, we showed that the over-expression of miR-362-5p enhanced the expression of IFN-γ, perforin, granzyme-B, and CD107a in human primary NK cells, and we found that silencing CYLD with a small interfering RNA (siRNA) mirrored the effect of miR-362-5p over-expression. In contrast, the inhibition of miR-362-5p had the opposite effect in NK cells, which was abrogated by CYLD siRNA, suggesting that miR-362-5p promotes NK-cell function, at least in part, by the down-regulation of CYLD. These results provide a resource for studying the roles of miRNAs in human NK cell biology and contribute to a better understanding of the physiologic significance of miRNAs in the regulation of NK cell function.

Indexed as

Transcriptome3' Untranslated RegionsBase SequenceCells, CulturedDeubiquitinating Enzyme CYLDDown-RegulationHEK293 CellsHumansKiller Cells, NaturalMicroRNAsRNA InterferenceRNA, Small InterferingSequence AlignmentTumor Suppressor Proteins3' Untranslated RegionsCYLD protein, humanDeubiquitinating Enzyme CYLDMicroRNAsRNA, Small InterferingTumor Suppressor Proteins

Identifiers

PMID25909817
PMCPMC4408982
OpenAlexW2099613172

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.