ArticleBMC genomics2015
Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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23 citing papers in PubMed, 44 citations in OpenAlex.
- Gut fungi of black-necked cranes (Grus nigricollis) respond to dietary changes during wintering.BMC microbiology · 2024Article
- Inducer-free cellulase production system based on the constitutive expression of mutated XYR1 and ACE3 in the industrial fungus Trichoderma reesei.Scientific reports · 2022Article
- Factors regulating cellulolytic gene expression in filamentous fungi: an overview.Microbial cell factories · 2022Review
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- Significant differences in intestinal fungal community of hooded cranes along the wintering periods.Frontiers in microbiology · 2022Article
- The phenomenon of degeneration of industrial Trichoderma reesei strains.Biotechnology for biofuels · 2021Article
- Genetic Transformation of Trichoderma spp.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Engineering the Effector Domain of the Artificial Transcription Factor to Improve Cellulase Production byFrontiers in bioengineering and biotechnology · 2020Article
- Rational engineering of theBiotechnology for biofuels · 2020Article
- The transcription factor ACE3 controls cellulase activities and lactose metabolism via two additional regulators in the fungusThe Journal of biological chemistry · 2019Article
- Use of fusion transcription factors to reprogram cellulase transcription and enable efficient cellulase production inBiotechnology for biofuels · 2019Article
- Protein hyperproduction in fungi by design.Applied microbiology and biotechnology · 2018Review
- Transcription Factor NsdD Regulates the Expression of Genes Involved in Plant Biomass-Degrading Enzymes, Conidiation, and Pigment Biosynthesis in Penicillium oxalicum.Applied and environmental microbiology · 2018Article
- Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi.Applied microbiology and biotechnology · 2018Review
- Enhanced cellulase production in Trichoderma reesei RUT C30 via constitution of minimal transcriptional activators.Microbial cell factories · 2018Article
- Primers and copper responsive promoter design and data of real-time RT-PCR assay in filamentous fungusData in brief · 2018Article
- The Promoter Toolbox for Recombinant Gene Expression inFrontiers in bioengineering and biotechnology · 2018Review
- Construction of enhanced transcriptional activators for improving cellulase production inBioresources and bioprocessing · 2018Article
- The transcription factor TpRfx1 is an essential regulator of amylase and cellulase gene expression inBiotechnology for biofuels · 2018Article
- Genome sequencing and transcriptome analysis ofBiotechnology for biofuels · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundTrichoderma reesei is the main industrial source of cellulases and hemicellulases required for the hydrolysis of biomass to simple sugars, which can then be used in the production of biofuels and biorefineries. The highly productive strains in use today were generated by classical mutagenesis. As byproducts of this procedure, mutants were generated that turned out to be unable to produce cellulases. In order to identify the mutations responsible for this inability, we sequenced the genome of one of these strains, QM9136, and compared it to that of its progenitor T. reesei QM6a.
resultsIn QM9136, we detected a surprisingly low number of mutagenic events in the promoter and coding regions of genes, i.e. only eight indels and six single nucleotide variants. One of these indels led to a frame-shift in the Zn₂Cys₆ transcription factor XYR1, the general regulator of cellulase and xylanase expression, and resulted in its C-terminal truncation by 140 amino acids. Retransformation of strain QM9136 with the wild-type xyr1 allele fully recovered the ability to produce cellulases, and is thus the reason for the cellulase-negative phenotype. Introduction of an engineered xyr1 allele containing the truncating point mutation into the moderate producer T. reesei QM9414 rendered this strain also cellulase-negative. The correspondingly truncated XYR1 protein was still able to enter the nucleus, but failed to be expressed over the basal constitutive level.
conclusionThe missing 140 C-terminal amino acids of XYR1 are therefore responsible for its previously observed auto-regulation which is essential for cellulases to be expressed. Our data present a working example of the use of genome sequencing leading to a functional explanation of the QM9136 cellulase-negative phenotype.
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