Evidence map›Paper›PMID 25909029›Full record

ArticleBiomedical optics express2015

Long-term two-photon neuroimaging with a photostable AIE luminogen.

Jun Qian, Zhenfeng Zhu, Chris Wai Tung Leung, Wang Xi, Liling Su, Guangdi Chen, Anjun Qin, Ben Zhong Tang, Sailing He

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jun QianState Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies; JORCEP (Sino-Swedish Joint Research Center of Photonics), Zhejiang University, 310058 Hangzhou, China.
Zhenfeng ZhuState Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies; JORCEP (Sino-Swedish Joint Research Center of Photonics), Zhejiang University, 310058 Hangzhou, China.
Chris Wai Tung LeungDepartment of Chemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Wang XiDepartment of Neurobiology, School of Medicine, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Liling SuBioelectromagnetics Laboratory, School of Medicine, Zhejiang University, Hangzhou 310058, China.
Guangdi ChenBioelectromagnetics Laboratory, School of Medicine, Zhejiang University, Hangzhou 310058, China.
Anjun QinSCUT-HKUST Joint Research Laboratory, Guangdong Innovative Research Team, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology (SCUT), Guangzhou 510640, China.
Ben Zhong TangDepartment of Chemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China ; SCUT-HKUST Joint Research Laboratory, Guangdong Innovative Research Team, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology (SCUT), Guangzhou 510640, China.
Sailing HeState Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies; JORCEP (Sino-Swedish Joint Research Center of Photonics), Zhejiang University, 310058 Hangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In neuroscience, fluorescence labeled two-photon microscopy is a promising tool to visualize ex vivo and in vivo tissue morphology, and track dynamic neural activities. Specific and highly photostable fluorescent probes are required in this technology. However, most fluorescent proteins and organic fluorophores suffer from photobleaching, so they are not suitable for long-term imaging and observation. To overcome this problem, we utilize tetraphenylethene-triphenylphosphonium (TPE-TPP), which possesses aggregation-induced emission (AIE) and two-photon fluorescence characteristics, for neuroimaging. The unique AIE feature of TPE-TPP makes its nanoaggregates resistant to photobleaching, which is useful to track neural cells and brain-microglia for a long period of time. Two-photon fluorescence of TPE-TPP facilitates its application in deep in vivo neuroimaging, as demonstrated in the present paper.

Indexed as

(160.2540) Fluorescent and luminescent materials(160.4890) Organic materials(170.3880) Medical and biological imaging(180.2520) Fluorescence microscopy(180.4315) Nonlinear microscopy(190.4180) Multiphoton processes

Identifiers

PMID25909029
PMCPMC4399684

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.