ArticleJournal of cell science2015
ENTH and ANTH domain proteins participate in AP2-independent clathrin-mediated endocytosis.
Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- The conserved protein adaptors CALM/AP180 and FCHo1/2 cooperatively recruit Eps15 to promote the initiation of clathrin-mediated endocytosis in yeast.PLoS biology · 2024Article
- Drug resistance in animal trypanosomiases: Epidemiology, mechanisms and control strategies.International journal for parasitology. Drugs and drug resistance · 2024Review
- The myxozoansFrontiers in cellular and infection microbiology · 2024Article
- Lessons from the deep: mechanisms behind diversification of eukaryotic protein complexes.Biological reviews of the Cambridge Philosophical Society · 2023Article
- Coarse-Grained Simulations Suggest Potential Competing Roles of Phosphoinositides and Amphipathic Helix Structures in Membrane Curvature Sensing of the AP180 N-Terminal Homology Domain.The journal of physical chemistry. B · 2022Article
- Article
- To the Surface and Back: Exo- and Endocytic Pathways inFrontiers in cell and developmental biology · 2021Review
- A nanobody-based molecular toolkit provides new mechanistic insight into clathrin-coat initiation.eLife · 2019Article
- Computational analysis of functional SNPs in Alzheimer's disease-associated endocytosis genes.PeerJ · 2019Article
- Evolutionary origins and specialisation of membrane transport.Current opinion in cell biology · 2018Review
- Membrane Curvature Sensing by Amphipathic Helices: Insights from Implicit Membrane Modeling.Biophysical journal · 2018Article
- Article
- Kingdom Chromista and its eight phyla: a new synthesis emphasising periplastid protein targeting, cytoskeletal and periplastid evolution, and ancient divergences.Protoplasma · 2018Article
- Structure and evolution of ENTH and VHS/ENTH-like domains in tepsin.Traffic (Copenhagen, Denmark) · 2017Article
- Lineage-specific proteins essential for endocytosis in trypanosomes.Journal of cell science · 2017Article
- Anti-trypanosomatid drug discovery: an ongoing challenge and a continuing need.Nature reviews. Microbiology · 2017Review
- Membrane Proteomic Insights into the Physiology and Taxonomy of an Oleaginous Green Microalga.Plant physiology · 2017Article
- Exploiting the Achilles' heel of membrane trafficking in trypanosomes.Current opinion in microbiology · 2016Review
- Blocking Synthesis of the Variant Surface Glycoprotein Coat in Trypanosoma brucei Leads to an Increase in Macrophage Phagocytosis Due to Reduced Clearance of Surface Coat Antibodies.PLoS pathogens · 2016Article
- Conservation and divergence within the clathrin interactome of Trypanosoma cruzi.Scientific reports · 2016Article
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4 authors.
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Abstract
Clathrin-mediated endocytosis (CME) is a major route of entry into eukaryotic cells. A core of evolutionarily ancient genes encodes many components of this system but much of our mechanistic understanding of CME is derived from a phylogenetically narrow sampling of a few model organisms. In the parasite Trypanosoma brucei, which is distantly related to the better characterised animals and fungi, exceptionally fast endocytic turnover aids its evasion of the host immune system. Although clathrin is absolutely essential for this process, the adaptor protein complex 2 (AP2) has been secondarily lost, suggesting mechanistic divergence. Here, we characterise two phosphoinositide-binding monomeric clathrin adaptors, T. brucei (Tb)EpsinR and TbCALM, which in trypanosomes are represented by single genes, unlike the expansions present in animals and fungi. Depletion of these gene products reveals essential, but partially redundant, activities in CME. Ultrastructural analysis of TbCALM and TbEpsinR double-knockdown cells demonstrated severe defects to clathrin-coated pit formation and morphology associated with a dramatic inhibition of endocytosis. Depletion of TbCALM alone, however, produced a distinct lysosomal segregation phenotype, indicating an additional non-redundant role for this protein. Therefore, TbEpsinR and TbCALM represent ancient phosphoinositide-binding proteins with distinct and vital roles in AP2-independent endocytosis.
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