ArticleExperimental biology and medicine (Maywood, N.J.)2015
Cytoskeletal prestress regulates nuclear shape and stiffness in cardiac myocytes.
Article in Experimental biology and medicine (Maywood, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Structural and functional benchmarking of monolayer- and bioreactor-generated hiPSC-derived cardiomyocytes.APL bioengineering · 2026Article
- Cytoskeletal prestress homeostasis is a biological principle that governs living cell structure and function.Mechanobiology in medicine · 2026Review
- Cardiomyocyte Nuclear Pleomorphism in a Mouse Model of Inherited Hypertrophic Cardiomyopathy.Journal of cardiovascular development and disease · 2025Article
- Microtubule forces drive nuclear damage in LMNA cardiomyopathy.Nature cardiovascular research · 2025Article
- MechanoBase: a comprehensive database for the mechanics of tissues and cells.Database : the journal of biological databases and curation · 2024Article
- Self-organizing behaviors of cardiovascular cells on synthetic nanofiber scaffolds.APL bioengineering · 2023Article
- Spatiotemporal cell junction assembly in human iPSC-CM models of arrhythmogenic cardiomyopathy.Stem cell reports · 2023Article
- Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles.Nature materials · 2023Article
- Soft bioelectronics for cardiac interfaces.Biophysics reviews · 2022Review
- Pathogenic Mechanisms of Hypertrophic Cardiomyopathy beyond Sarcomere Dysfunction.International journal of molecular sciences · 2021Review
- Nuclear Stiffness Decreases with Disruption of the Extracellular Matrix in Living Tissues.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- Mitochondrial architecture in cardiac myocytes depends on cell shape and matrix rigidity.Journal of molecular and cellular cardiology · 2021Article
- Matrix Stiffening Enhances DNCB-Induced IL-6 Secretion in Keratinocytes Through Activation of ERK and PI3K/Akt Pathway.Frontiers in immunology · 2021Article
- TENSCell: Imaging of Stretch-Activated Cells Reveals Divergent Nuclear Behavior and Tension.Biophysical journal · 2020Article
- Nuclear Mechanics within Intact Cells Is Regulated by Cytoskeletal Network and Internal Nanostructures.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Human brain microvascular endothelial cell pairs model tissue-level blood-brain barrier function.Integrative biology : quantitative biosciences from nano to macro · 2020Article
- Cellular Aging Characteristics and Their Association with Age-Related Disorders.Antioxidants (Basel, Switzerland) · 2020Review
- Recapitulation of molecular regulators of nuclear motion during cell migration.Cell adhesion & migration · 2019Review
- Regulation of nuclear architecture, mechanics, and nucleocytoplasmic shuttling of epigenetic factors by cell geometric constraints.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Effect of Mechanical Stretch on the DNCB-induced Proinflammatory Cytokine Secretion in Human Keratinocytes.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Mechanical stresses on the myocyte nucleus have been associated with several diseases and potentially transduce mechanical stimuli into cellular responses. Although a number of physical links between the nuclear envelope and cytoplasmic filaments have been identified, previous studies have focused on the mechanical properties of individual components of the nucleus, such as the nuclear envelope and lamin network. The mechanical interaction between the cytoskeleton and chromatin on nuclear deformability remains elusive. Here, we investigated how cytoskeletal and chromatin structures influence nuclear mechanics in cardiac myocytes. Rapid decondensation of chromatin and rupture of the nuclear membrane caused a sudden expansion of DNA, a consequence of prestress exerted on the nucleus. To characterize the prestress exerted on the nucleus, we measured the shape and the stiffness of isolated nuclei and nuclei in living myocytes during disruption of cytoskeletal, myofibrillar, and chromatin structure. We found that the nucleus in myocytes is subject to both tensional and compressional prestress and its deformability is determined by a balance of those opposing forces. By developing a computational model of the prestressed nucleus, we showed that cytoskeletal and chromatin prestresses create vulnerability in the nuclear envelope. Our studies suggest the cytoskeletal-nuclear-chromatin interconnectivity may play an important role in mechanics of myocyte contraction and in the development of laminopathies by lamin mutations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.