ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2015
Identification and visualization of multidimensional antigen-specific T-cell populations in polychromatic cytometry data.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Innate immune imprints shape HIV-1 reservoir cell persistence during long-term antiretroviral therapy.Nature immunology · 2026Article
- cytoGPNet: Enhancing clinical outcome prediction accuracy using longitudinal cytometry data in small cohort studies.Patterns (New York, N.Y.) · 2025Article
- IL-10 normalizes aberrant amygdala GABA transmission and reverses anxiety-like behavior and dependence-induced escalation of alcohol intake.Progress in neurobiology · 2021Article
- Generation of highly activated, antigen-specific tumor-infiltrating CD8Oncoimmunology · 2020Article
- Review
- Article
- Automated optimized parameters for T-distributed stochastic neighbor embedding improve visualization and analysis of large datasets.Nature communications · 2019Article
- CytoML for cross-platform cytometry data sharing.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Article
- Single cell analysis of Crohn's disease patient-derived small intestinal organoids reveals disease activity-dependent modification of stem cell properties.Journal of gastroenterology · 2018Article
- Characterization of T-cell immune responses in clinical trials of the candidate RTS,S malaria vaccine.Human vaccines & immunotherapeutics · 2018Review
- Article
- Comparative exploration of multidimensional flow cytometry software: a model approach evaluating T cell polyfunctional behavior.Journal of leukocyte biology · 2017Article
- Using Visualization ofJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- CyTOF workflow: differential discovery in high-throughput high-dimensional cytometry datasets.F1000Research · 2017Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
An important aspect of immune monitoring for vaccine development, clinical trials, and research is the detection, measurement, and comparison of antigen-specific T-cells from subject samples under different conditions. Antigen-specific T-cells compose a very small fraction of total T-cells. Developments in cytometry technology over the past five years have enabled the measurement of single-cells in a multivariate and high-throughput manner. This growth in both dimensionality and quantity of data continues to pose a challenge for effective identification and visualization of rare cell subsets, such as antigen-specific T-cells. Dimension reduction and feature extraction play pivotal role in both identifying and visualizing cell populations of interest in large, multi-dimensional cytometry datasets. However, the automated identification and visualization of rare, high-dimensional cell subsets remains challenging. Here we demonstrate how a systematic and integrated approach combining targeted feature extraction with dimension reduction can be used to identify and visualize biological differences in rare, antigen-specific cell populations. By using OpenCyto to perform semi-automated gating and features extraction of flow cytometry data, followed by dimensionality reduction with t-SNE we are able to identify polyfunctional subpopulations of antigen-specific T-cells and visualize treatment-specific differences between them.
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