Evidence map›Paper›PMID 25907253›Full record

ArticleMethods (San Diego, Calif.)2015

Rapid and comprehensive 'shotgun' lipidome profiling of colorectal cancer cell derived exosomes.

Todd A Lydic, Steven Townsend, Christopher G Adda, Christine Collins, Suresh Mathivanan, Gavin E Reid

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods (San Diego, Calif.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.

0numbers the graph read from it
0cells of the map it votes in
113citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

113 citing papers in PubMed, 185 citations in OpenAlex.

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  20. Metabolic features of tumor-derived extracellular vesicles: challenges and opportunities.Extracellular vesicles and circulating nucleic acids · 2024
    Review

53 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Todd A LydicDepartment of Physiology, Michigan State University, East Lansing, MI 48824, USA.
Steven TownsendDepartment of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
Christopher G AddaDepartment of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
Christine CollinsDepartment of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
Suresh MathivananDepartment of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
Gavin E ReidDepartment of Chemistry, Michigan State University, East Lansing, MI 48824, USA; School of Chemistry, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: gavin.reid@unimelb.edu.au.
La Trobe University · AUMichigan State University · USUniversity of Melbourne · AU

Funding

Mass Spectrometry Strategies to Identify Lipid Biomarkers of DiseaseR01GM103508 · NIGMS · MICHIGAN STATE UNIVERSITY · PI REID, GAVIN EDMUND · 2012 to 2012
$335k
NIGMS NIH HHS GM103508NIGMS NIH HHS R01 GM103508
6 · The paper itself

Abstract

There is an increasing recognition of the role that cancer cell derived exosomes play in intercellular signaling upon fusion or uptake with a target cell, including immune system evasion, tumor growth and metastasis. To date, however, although exosomal membrane and cargo lipids are expected to play a pivotal role in exosome biogenesis and secretion, as well as in fusion or uptake and target cell functional response, the detailed characterization of cancer cell derived exosome lipids across a range of different cancers has not yet been broadly explored. Here, a simple and straightforward lipidome analysis strategy consisting of optimized sample extraction and novel sample derivatization techniques, coupled with high-resolution 'shotgun' mass spectrometry and 'targeted' tandem mass spectrometry methods, is demonstrated for the rapid identification of >520 individual lipids in 36 lipid classes and sub classes from exosomes secreted by the colorectal cancer cell line, LIM1215. Relative quantification and comparison of exosome versus cellular lipid profiles reveals significant enrichment of certain lipid classes, as well as substantial lipid subclass remodeling and changes in abundance of individual lipids, including sphingolipids, sterol lipids, glycerolipids and glycerophospholipids, and particularly plasmalogen- and alkyl ether-containing glycerophospholipids. This analysis strategy therefore provides a platform for comprehensive lipidome profiling across a wide range of cancer cell or tissue derived exosomes, that will facilitate subsequent functional studies aimed at elucidating the role of specific cellular or exosome lipids in the onset and progression of colorectal cancer, or to identify specific lipid(s) that could serve as effective diagnostic or prognostic disease biomarkers.

Indexed as

Cell FractionationCell Line, TumorCholesterolEpithelial CellsExosomesFatty AcidsGlycerophospholipidsHumansLiquid Phase MicroextractionMetabolomeRectumSphingolipidsTandem Mass SpectrometryCholesterolFatty AcidsGlycerophospholipidsSphingolipidsColorectal cancerExosomeLipidomeMass spectrometry

Identifiers

PMID25907253
PMCPMC4615275
OpenAlexW2025612331

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.