ArticleBMC microbiology2015
Identification and functional characterization of the CYP51 gene from the yeast Xanthophyllomyces dendrorhous that is involved in ergosterol biosynthesis.
Article in BMC microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 25 citations in OpenAlex.
- Genome-Wide Characterization and Expression Analysis ofBiology · 2025Article
- Salicylic acid-induced upregulation of AtAACT and AtMVD expression enhances triterpene biosynthesis in Athelia termitophila.World journal of microbiology & biotechnology · 2025Article
- Damage response protein 1 (Dap1) functions in the synthesis of carotenoids and sterols in Xanthophyllomyces dendrorhous.Journal of lipid research · 2022Article
- Deciphering the mechanism by which the yeast Phaffia rhodozyma responds adaptively to environmental, nutritional, and genetic cues.Journal of industrial microbiology & biotechnology · 2021Review
- Three-Component Synthesis of 2-Amino-3-cyano-4Pharmaceuticals (Basel, Switzerland) · 2021Article
- The SREBP (Sterol Regulatory Element-Binding Protein) pathway: a regulatory bridge between carotenogenesis and sterol biosynthesis in the carotenogenic yeast Xanthophyllomyces dendrorhous.Biological research · 2021Review
- Sterol regulatory element-binding protein Sre1 regulates carotenogenesis in the red yeastJournal of lipid research · 2020Article
- Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (Animals : an open access journal from MDPI · 2020Article
- Metallopeptidase Stp1 activates the transcription factor Sre1 in the carotenogenic yeastJournal of lipid research · 2020Article
- Phenotypic Analysis of Mutants of Ergosterol Biosynthesis Genes (Frontiers in microbiology · 2020Article
- Sterol Regulatory Element-Binding Protein (Sre1) Promotes the Synthesis of Carotenoids and Sterols inFrontiers in microbiology · 2019Article
- Comparative analyses and structural insights of the novel cytochrome P450 fusion protein family CYP5619 in Oomycetes.Scientific reports · 2018Article
- Characterization of the cytochrome P450 monooxygenase genes (P450ome) from the carotenogenic yeast Xanthophyllomyces dendrorhous.BMC genomics · 2017Article
- Functional characterization of thiolase-encoding genes from Xanthophyllomyces dendrorhous and their effects on carotenoid synthesis.BMC microbiology · 2016Article
- Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: the role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression.Microbial cell factories · 2016Article
- Article
- Ergosterol Alleviates Kidney Injury in Streptozotocin-Induced Diabetic Mice.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundXanthophyllomyces dendrorhous is a basidiomycetous yeast that synthesizes astaxanthin, a carotenoid with great biotechnological impact. The ergosterol and carotenoid synthetic pathways derive from the mevalonate pathway and involve cytochrome P450 enzymes. Among these enzymes, the CYP51 family, which is involved in ergosterol biosynthesis, is one of the most remarkable that has C14-demethylase activity.
resultsIn this study, the CYP51 gene from X. dendrorhous was isolated and its function was analyzed. The gene is composed of ten exons and encodes a predicted 550 amino acid polypeptide that exhibits conserved cytochrome P450 structural characteristics and shares significant identity with the sterol C14-demethylase from other fungi. The functionality of this gene was confirmed by heterologous complementation in S. cerevisiae. Furthermore, a CYP51 gene mutation in X. dendrorhous reduced sterol production by approximately 40% and enhanced total carotenoid production by approximately 90% compared to the wild-type strain after 48 and 120 h of culture, respectively. Additionally, the CYP51 gene mutation in X. dendrorhous increased HMGR (hydroxy-methylglutaryl-CoA reductase, involved in the mevalonate pathway) and crtR (cytochrome P450 reductase) transcript levels, which could be associated with reduced ergosterol production.
conclusionsThese results suggest that the CYP51 gene identified in X. dendrorhous encodes a functional sterol C14-demethylase that is involved in ergosterol biosynthesis.
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