Evidence map›Paper›PMID 25906946›Full record

ArticleBiochimica et biophysica acta2015

Platelet membrane variations and their effects on δ-granule secretion kinetics and aggregation spreading among different species.

Sarah M Gruba, Secil Koseoglu, Audrey F Meyer, Ben M Meyer, Melissa A Maurer-Jones, Christy L Haynes

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. The role of cholesterol in membrane fusion.Chemistry and physics of lipids · 2016
    Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Sarah M GrubaDepartment of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455, USA.
Secil KoseogluDepartment of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455, USA.
Audrey F MeyerDepartment of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455, USA.
Ben M MeyerDepartment of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455, USA.
Melissa A Maurer-JonesDepartment of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455, USA.
Christy L HaynesDepartment of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455, USA. Electronic address: chaynes@umn.edu.
University of Minnesota · US

Funding

TRAINING FOR FUTURE BIOTECHNOLOGY DEVELOPMENTT32GM008347 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI SCHMIDT-DANNERT, CLAUDIA · 1990 to 2021
$11.0M
IMMUNE SYSTEM-ON-A-CHIP FOR QUANTITATIVE ANALYSIS OF CELL INTERACTIONS DURING ALLDP2OD004258 · OD · UNIVERSITY OF MINNESOTA · PI HAYNES, CHRISTY L · 2008 to 2008
$2.3M
NIGMS NIH HHS 5T32GM008347-23NIH HHS DP2 OD004258
6 · The paper itself

Abstract

Platelet exocytosis is regulated partially by the granular/cellular membrane lipids and proteins. Some platelets contain a membrane-bound tube, called an open canalicular system (OCS), which assists in granular release events and increases the membrane surface area for greater spreading. The OCS is not found in all species, and variations in membrane composition can cause changes in platelet secretion. Since platelet studies use various animal models, it is important to understand how platelets differ in both their composition and granular release to draw conclusions among various models. The relative phospholipid composition of the platelets with (mouse, rabbit) and without (cow) an OCS was quantified using UPLC-MS/MS. Cholesterol and protein composition was measured using an Amplex Red Assay and BCA Assay. TEM and dark field platelet images were gathered and analyzed with Image J. Granular release was monitored with single cell carbon fiber microelectrode amperometry. Cow platelets contained greater amounts of cholesterol and sphingomyelin. In addition, they yield greater serotonin release and longer δ granule secretion times. Finally, they showed greater spreading area with a greater range of spread. Platelets containing an OCS had more similarities in their membrane composition and secretion kinetics compared to cow platelets. However, cow platelets showed greater fusion pore stability which could be due to extra sphingomyelin and cholesterol, the primary components of lipid rafts. In addition, their greater stability may lead to many granules assisting in spreading. This study highlights fundamental membrane differences and their effects on platelet secretion.

Indexed as

Platelet AggregationAnimalsBlood PlateletsCarbonCarbon FiberCattleCell MembraneCholesterolChromatography, High Pressure LiquidElectrochemical TechniquesExocytosisKineticsMembrane LipidsMembrane ProteinsMiceMicroelectrodesCarbonCarbon FiberCholesterolMembrane LipidsMembrane ProteinsExocytosisOpen canalicular systemPhospholipidsPlatelets

Identifiers

PMID25906946
PMCPMC4431631
OpenAlexW2081258085

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.