ArticleJournal of endodontics2015
Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing.
Article in Journal of endodontics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 113 citations in OpenAlex.
- Elimination of Enterococcus faecalis from root canal system using laser-activated nanoparticles: a systematic review.Lasers in medical science · 2023Pooled it
- Clinical and microbiological effectiveness of photodynamic therapy on primary endodontic infections: a 6-month randomized clinical trial.Clinical oral investigations · 2018Trial
- Root canal microbiota in diabetic versus non-diabetic patients with apical periodontitis: A systematic review.The Japanese dental science review · 2026Review
- Association Between the Root Canal Microbiome and Apical Lesion Size: An Observational Shotgun Metagenomic Study.International endodontic journal · 2026Observational
- A Comparison of Endodontic Microbiomes Associated With Symptomatic and Asymptomatic Apical Periodontitis by Next-Generation Sequencing.International endodontic journal · 2026Article
- Is apical periodontitis a matter of microbial diversity or time? A scoping review.Head & face medicine · 2026Article
- Distinct bacteria profiles in primary and secondary/persistent endodontic infections: a 16S rRNA gene sequencing study.BMC oral health · 2026Article
- Profiling of microbes associated with chronic irreversible pulpitis using metagenomic next-generation sequencing.BMC oral health · 2025Article
- Comparative Analysis of Root Canal Microbiota in Patients with Diabetes and Systemically Healthy Individuals: A Pilot Next-Generation Sequencing Study.Journal of clinical medicine · 2025Article
- Primary and Re-exposure effects of D-enantiomeric peptide on metabolism, diversity, and composition of oral biofilms at different stages of recovery.Bioactive materials · 2025Article
- A polymicrobial perspective into the ecological role of Enterococcus faecalis in dental root canal infections.NPJ biofilms and microbiomes · 2025Review
- Analysis of the diversity and function of the microbiota in infected root canals of type 2 diabetes mellitus with apical periodontitis.Frontiers in cellular and infection microbiology · 2025Article
- Microbial Dynamics in Endodontic Pathology-From Bacterial Infection to Therapeutic Interventions-A Narrative Review.Pathogens (Basel, Switzerland) · 2024Review
- Microbial diversity in primary endodontic infections: demographics and radiographic characteristics.Clinical oral investigations · 2024Article
- Establishment of a Protocol for Viability qPCR in Dental Hard Tissues.Microorganisms · 2024Article
- Article
- Dental pathologies of endodontic origin and subsequent bacterial involvement - a literature review.Germs · 2023Review
- Antibacterial and Biofilm Production Inhibition Activity ofPathogens (Basel, Switzerland) · 2023Article
- Amplicon-based next-generation sequencing for comparative analysis of root canal microbiome of teeth with primary and persistent/secondary endodontic infections.Clinical oral investigations · 2023Article
- Taxonomic abundance in primary and secondary root canal infections.International endodontic journal · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
introductionElucidating the microbial ecology of endodontic infections (EIs) is a necessary step in developing effective intracanal antimicrobials. The aim of the present study was to investigate the bacterial composition of symptomatic and asymptomatic primary and persistent infections in a Greek population using high-throughput sequencing methods.
methods16S amplicon pyrosequencing of 48 root canal bacterial samples was conducted, and sequencing data were analyzed using an oral microbiome-specific and a generic (Greengenes) database. Bacterial abundance and diversity were examined by EI type (primary or persistent), and statistical analysis was performed by using non-parametric and parametric tests accounting for clustered data.
resultsBacteroidetes was the most abundant phylum in both infection groups. Significant, albeit weak associations of bacterial diversity were found, as measured by UniFrac distances with infection type (analyses of similarity, R = 0.087, P = .005) and symptoms (analyses of similarity, R = 0.055, P = .047). Persistent infections were significantly enriched for Proteobacteria and Tenericutes compared with primary ones; at the genus level, significant differences were noted for 14 taxa, including increased enrichment of persistent infections for Lactobacillus, Streptococcus, and Sphingomonas. More but less abundant phyla were identified using the Greengenes database; among those, Cyanobacteria (0.018%) and Acidobacteria (0.007%) were significantly enriched among persistent infections. Persistent infections showed higher phylogenetic diversity (PD) (asymptomatic: PD = 9.2, standard error [SE] = 1.3; symptomatic: PD = 8.2, SE = 0.7) compared with primary infections (asymptomatic: PD = 5.9, SE = 0.8; symptomatic: PD = 7.4, SE = 1.0).
conclusionsThe present study revealed a high bacterial diversity of EI and suggests that persistent infections may have more diverse bacterial communities than primary infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.