ArticlePloS one2015
Transcriptional and Phenotypic Characterization of Novel Spx-Regulated Genes in Streptococcus mutans.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 38 citations in OpenAlex.
- Normoxic stress enhance the secretion of adhesion-promoting membrane vesicles byJournal of oral microbiology · 2026Article
- Synergistic inhibition ofFrontiers in microbiology · 2026Article
- Deciphering the role of SMU.1147 in peptide-mediated signaling and competence inMicrobiology spectrum · 2025Article
- Natural compounds: new therapeutic approach for inhibition ofFrontiers in pharmacology · 2025Review
- Oxidative stress response: a critical factor affecting the ecological competitiveness ofJournal of oral microbiology · 2024Review
- Multiple factors regulate the expression ofFrontiers in cellular and infection microbiology · 2024Article
- Metal Homeostasis in Pathogenic Streptococci.Microorganisms · 2022Review
- TheMicrobiology spectrum · 2022Article
- Disruption of theJournal of bacteriology · 2022Article
- Redox Sensing Modulates the Activity of the ComE Response Regulator of Streptococcus mutans.Journal of bacteriology · 2021Article
- Mechanistic insights into the inhibitory effect of theaflavins on virulence factors production in Streptococcus mutans.AMB Express · 2021Article
- Regulatory involvement of the PerR and SloR metalloregulators in theJournal of bacteriology · 2021Article
- Article
- The Cytoplasmic Domains ofFrontiers in microbiology · 2021Article
- Identification of conditionally essential genes forVirulence · 2020Article
- Regulatory circuits controlling Spx levels in Streptococcus mutans.Molecular microbiology · 2020Article
- The Biology ofMicrobiology spectrum · 2019Article
- Disruption of a Novel Iron Transport System Reverses Oxidative Stress Phenotypes of aJournal of bacteriology · 2018Article
- Transcriptome responses of Streptococcus mutans to peroxide stress: identification of novel antioxidant pathways regulated by Spx.Scientific reports · 2017Article
- Inactivation of the spxA1 or spxA2 gene of Streptococcus mutans decreases virulence in the rat caries model.Molecular oral microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
In oral biofilms, two of the major environmental challenges encountered by the dental pathogen Streptococcus mutans are acid and oxidative stresses. Previously, we showed that the S. mutans transcriptional regulators SpxA1 and SpxA2 (formerly SpxA and SpxB, respectively) are involved in stress survival by activating the expression of classic oxidative stress genes such as dpr, nox, sodA and tpx. We reasoned that some of the uncharacterized genes under SpxA1/A2 control are potentially involved in oxidative stress management. Therefore, the goal of this study was to use Spx-regulated genes as a tool to identify novel oxidative stress genes in S. mutans. Quantitative real-time PCR was used to evaluate the responses of ten Spx-regulated genes during H2O2 stress in the parent and Δspx strains. Transcription activation of the H2O2-induced genes (8 out of 10) was strongly dependent on SpxA1 and, to a lesser extent, SpxA2. In vitro transcription assays revealed that one or both Spx proteins directly regulate three of these genes. The gene encoding the FeoB ferrous permease was slightly repressed by H2O2 but constitutively induced in strains lacking SpxA1. Nine genes were selected for downstream mutational analysis but inactivation of smu127, encoding a subunit of the acetoin dehydrogenase was apparently lethal. In vitro and in vivo characterization of the viable mutants indicated that, in addition to the transcriptional activation of reducing and antioxidant pathways, Spx performs an important role in iron homeostasis by regulating the intracellular availability of free iron. In particular, inactivation of the genes encoding the Fe-S biogenesis SUF system and the previously characterized iron-binding protein Dpr resulted in impaired growth under different oxidative stress conditions, increased sensitivity to iron and lower infectivity in rats. These results serve as an entryway into the characterization of novel genes and pathways that allow S. mutans to cope with oxidative stress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.