ArticleScientific reports2015
Mechanistic insights into elastin degradation by pseudolysin, the major virulence factor of the opportunistic pathogen Pseudomonas aeruginosa.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 46 citations in OpenAlex.
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- The Etiological and Antimicrobial Susceptibility Profiles of the Bacteria Obtained from Ovine Caseous Lymphadenitis Cases in the Çankırı Region, Türkiye.Life (Basel, Switzerland) · 2024Article
- The role of human extracellular matrix proteins in defining Staphylococcus aureus biofilm infections.FEMS microbiology reviews · 2024Review
- Examining the influence of environmental factors on Acanthamoeba castellanii and Pseudomonas aeruginosa in co-culture.PloS one · 2024Article
- Novel marine metalloprotease-new approaches for inhibition of biofilm formation of Stenotrophomonas maltophilia.Applied microbiology and biotechnology · 2023Article
- An anti-biofilm cyclic peptide targets a secreted aminopeptidase from P. aeruginosa.Nature chemical biology · 2023Article
- A step forward to the optimized HlyA type 1 secretion system through directed evolution.Applied microbiology and biotechnology · 2023Article
- Glucose-Induced Enhanced Virulence in Strains of Multidrug-Resistant Pseudomonas aeruginosa Isolated from Diabetic Patients.Current microbiology · 2023Article
- Protein sociology of ProA, Mip and other secreted virulence factors at theFrontiers in cellular and infection microbiology · 2023Review
- A survey of elastase-producing bacteria and characteristics of the most potent producer, Priestia megaterium gasm32.PloS one · 2023Article
- The matricellular protein thrombospondin-1 in lung inflammation and injury.American journal of physiology. Cell physiology · 2022Review
- Synthesis and antimicrobial activity ofRussian chemical bulletin = Izvestiia Akademii nauk. Seriia khimicheskaia · 2022Article
- Antibiogram profile and virulence signatures of Pseudomonas aeruginosa isolates recovered from selected agrestic hospital effluents.Scientific reports · 2021Article
- GroEL protein of the Leptospira spp. interacts with host proteins and induces cytokines secretion on macrophages.BMC microbiology · 2021Article
- Virtual Screening Approach to Identifying a Novel and Tractable Series ofACS medicinal chemistry letters · 2021Article
- Pseudomonas aeruginosa virulence proteins pseudolysin and protease IV impede cutaneous wound healing.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- Article
- Detection of virulence and multidrug resistance operons inVeterinary world · 2019Article
- DisarmingFrontiers in microbiology · 2019Article
- A Novel Broad-Spectrum Elastase-Like Serine Protease From the Predatory BacteriumFrontiers in microbiology · 2019Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudolysin is the most abundant protease secreted by Pseudomonas aeruginosa and is the major extracellular virulence factor of this opportunistic human pathogen. Pseudolysin destroys human tissues by solubilizing elastin. However, the mechanisms by which pseudolysin binds to and degrades elastin remain elusive. In this study, we investigated the mechanism of action of pseudolysin on elastin binding and degradation by biochemical assay, microscopy and site-directed mutagenesis. Pseudolysin bound to bovine elastin fibers and preferred to attack peptide bonds with hydrophobic residues at the P1 and P1' positions in the hydrophobic domains of elastin. The time-course degradation processes of both bovine elastin fibers and cross-linked human tropoelastin by pseudolysin were further investigated by microscopy. Altogether, the results indicate that elastin degradation by pseudolysin began with the hydrophobic domains on the fiber surface, followed by the progressive disassembly of macroscopic elastin fibers into primary structural elements. Moreover, our site-directed mutational results indicate that five hydrophobic residues in the S1-S1' sub-sites played key roles in the binding of pseudolysin to elastin. This study sheds lights on the pathogenesis of P. aeruginosa infection.
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