ArticleIranian journal of parasitology
Morphological and molecular discrimination of fasciola species isolated from domestic ruminants of urmia city, iran.
Article in Iranian journal of parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran.Veterinary medicine and science · 2024Article
- First morphometric and molecular characterization of Fasciola spp. in Northwest Tunisia.Parasitology research · 2023Article
- Rapid Detection and Identification ofIranian journal of public health · 2023Article
- Comparison of polymerase chain reaction and microscopy for the detection ofVeterinary world · 2021Article
- Detection of Fasciola Hepatica in Lori Sheep Using Polymerase Chain Reaction and Conventional Diagnostic Methods in Western Iran.Archives of Razi Institute · 2021Article
- Molecular phylogenetic and genetic variability ofVeterinary research forum : an international quarterly journal · 2020Article
- Molecular and Morphometrical Characterization ofIranian journal of public health · 2017Article
- Identity of Fasciola spp. in sheep in Egypt.Parasites & vectors · 2016Article
- Molecular characterization ofVeterinary world · 2016Article
- Molecular Characterization ofIranian journal of parasitologyArticle
- Molecular Determination ofIranian journal of parasitologyArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe trematodes of the genus Fasciola (the liver flukes) are among the well-known instances of food-borne parasites worldwide. Differentiation of Fasciola species is important because of their different transmission and epidemiological characteristics. The current study was undertaken to discriminate Fasciola species in the domestic ruminants of Urmia city, Iran.
methodsAdult flukes were isolated from the naturally infected livers of the slaughtered water buffaloes and sheep. The flukes were initially identified based on morphological and morphometric parameters. A 618-bp-long fragment of the 28SrRNA gene of Fasciola was amplified by polymerase chain reaction (PCR). The amplified fragment was digested by DraII or AvaII enzymes for a restriction fragment length polymorphism (RFLP) analysis and sequenced for the phylogenetic tree construction.
resultsBased on the morphometric examination, the flukes belonged to F. hepatica, F. gigantica and an intermediate Fasciola form. The PCR-RFLP analysis was able to differentiate F. hepatica from F. gigantica. While the phylogenetic reconstruction justified, to some extent, the morphological diagnosis, it failed to segregate F. hepatica from F. gigantica identified in this and the previous studies.
conclusionTo resolve fully the problem of taxonomy and evolution in Fasciola species, employing a broad range of molecular and morphological approaches is necessary. This is crucial for epidemiological surveys and successful clinical management of their infection.
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25904945PMC4403539W2132325376What OpenQuestion holds
Registered trials
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