ArticleIranian journal of parasitology
Comparison of Proteome Profiling of Two Sensitive and Resistant Field Iranian Isolates of Leishmania major to Glucantime® by 2- Dimensional Electrophoresis.
Article in Iranian journal of parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 21 citations in OpenAlex.
- Comparison of the efficacy of local thermotherapy versus Glucantime® in the treatment of anthroponotic cutaneous leishmaniasis: a pilot study.Parasitology research · 2025Article
- Genetic disruption of nucleoside transporter 4 reveals its critical roles in malaria parasite sporozoite functions.Pathogens and global health · 2023Article
- Typical features of cutaneous leishmaniasis in the Ilam province, Iran.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2020Article
- Treatment Failure in Cutaneous Leishmaniasis Patients Referred to the School of Public Health, Tehran University of Medical Sciences during 2008-2017.Journal of arthropod-borne diseases · 2020Article
- Experimental Strategies to Explore Drug Action and Resistance in Kinetoplastid Parasites.Microorganisms · 2020Review
- Evaluation of MDR1 and MRPA genes expression in different types of dry cutaneous leishmaniasis.BMC research notes · 2019Article
- Comparative Two-dimensional Gel Electrophoresis Maps for Amastigote-like Proteomes of IranianGalen medical journal · 2019Article
- Investigation of Visceral Leishmaniasis among 192 Dog Carcasses Killed by Road Accidents in Khorasan Razavi, Northeastern Iran during 2014-2016.Iranian journal of public health · 2018Article
- Understanding Leishmania parasites through proteomics and implications for the clinic.Expert review of proteomics · 2018Review
- Antimicrobial activity of an antimicrobial peptide against amastigote forms ofVeterinary research forum : an international quarterly journal · 2018Article
- Molecular Characterization of Aquaglyceroporine: A Novel Mutation inIranian journal of parasitologyArticle
- A Novel Strategy for Enhance Potentiation of Meglumine antimoniate againstIranian journal of parasitologyArticle
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6 authors at 2 institutions in 1 country.
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Abstract
backgroundIn this study, two-dimensional gel electrophoresis (2-DE) method was applied to determine and compare the protein spots expressed in the two field isolates of Leishmania major and recovered from the patients who were clinically sensitive and resistant to Glucantime® treatment.
methodsLeishmania parasites were isolated from the cutaneous lesions of two CL infected patients in Shiraz, south of Iran. The species of the two isolates were identified as L. major using Nested-PCR. Sensitivity (Sh-214S) and resistance (Sh-120R) of the two isolates to meglumine antimonite were checked by the standard in vitro assays. Both sensitive and resistant L. major isolates were harvested in RPMI 1640 medium. Protein extractions were performed using TCA/Acetone method and the protein spots were determined by a two-dimensional gel electrophoresis (2-DE). The gels were stained with silver nitrate and analyzed by Image Master 2D Melanie-6 software.
resultsAbout 2967 protein spots were detected. Overall, 89 protein spots represented considerable changes of expression in the resistant isolate of L. major compared to the sensitive isolate. Of these, 60 and 29 protein spots were up-and down regulated, respectively. In addition, 11 protein spots present in the resistant isolate were noticed to be absent in the sensitive isolate.
conclusionA number of proteins showed significant changes of expression in the drug-resistant L. major; moreover, the roles of these proteins probably enhanced the parasite resistance to the drug and increased parasite survival in the cells.
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Identifiers
25904942PMC4403536W2097212379What OpenQuestion holds
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