ArticleMolecular biology of the cell2015
ABCF1 extrinsically regulates retinal pigment epithelial cell phagocytosis.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 1 citations in OpenAlex.
- Insights into Noncanonical and Diversified Functions of ABCF1: From Health to Disease.Journal of molecular biology · 2025Review
- Secretogranin III: a promising therapeutic target for intraocular neovascular lesions.International ophthalmology · 2025Review
- Secretogranin III Selectively Promotes Vascular Leakage in the Deep Vascular Plexus of Diabetic Retinopathy.International journal of molecular sciences · 2023Article
- Profiling disease-selective drug targets: From proteomics to ligandomics.Drug discovery today · 2023Review
- Platelet factor 4 improves survival in a murine model of antibiotic-susceptible and methicillin-resistantFrontiers in cellular and infection microbiology · 2023Article
- Pluripotent Stem Cells in Clinical Cell Transplantation: Focusing on Induced Pluripotent Stem Cell-Derived RPE Cell Therapy in Age-Related Macular Degeneration.International journal of molecular sciences · 2022Review
- The human ATP-binding cassette (ABC) transporter superfamily.Human mutation · 2022Review
- Genome-Wide Identification, Characterisation and Phylogenetic Analysis of 52 Striped Catfish (Tropical life sciences research · 2022Article
- ABC-F translation factors: from antibiotic resistance to immune response.FEBS letters · 2021Review
- Comparative Ligandomic Analysis of Human Lung Epithelial Cells Exposed to PMBiomedical and environmental sciences : BES · 2020Article
- Zika Infection Disrupts Proteins Involved in the Neurosensory System.Frontiers in cell and developmental biology · 2020Article
- Biochemical characterization of the mouse ABCF3 protein, a partner of the flavivirus-resistance protein OAS1B.The Journal of biological chemistry · 2019Article
- Association study identified biologically relevant receptor genes with synergistic functions in celiac disease.Scientific reports · 2019Article
- Ligandomics: a paradigm shift in biological drug discovery.Drug discovery today · 2018Review
- Secretogranin III: a diabetic retinopathy-selective angiogenic factor.Cellular and molecular life sciences : CMLS · 2018Review
- Clearance of Apoptotic Cells by Tissue Epithelia: A Putative Role for Hepatocytes in Liver Efferocytosis.Frontiers in immunology · 2018Review
- Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.The Journal of experimental medicine · 2017Article
- Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer.International journal of molecular sciences · 2017Article
- Mesd extrinsically promotes phagocytosis by retinal pigment epithelial cells.Cell biology and toxicology · 2016Article
- The regulatory role of hepatoma-derived growth factor as an angiogenic factor in the eye.Molecular vision · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Phagocytosis of shed photoreceptor outer segments (POSs) by retinal pigment epithelial (RPE) cells is critical to retinal homeostasis and shares many conserved signaling pathways with other phagocytes, including extrinsic regulations. Phagocytotic ligands are the key to cargo recognition, engulfment initiation, and activity regulation. In this study, we identified intracellular protein ATP-binding cassette subfamily F member 1 (ABCF1) as a novel RPE phagocytotic ligand by a new approach of functional screening. ABCF1 was independently verified to extrinsically promote phagocytosis of shed POSs by D407 RPE cells. This finding was further corroborated with primary RPE cells and RPE explants. Internalized POS vesicles were colocalized with a phagosome marker, suggesting that ABCF1-mediated engulfment is through a phagocytic pathway. ABCF1 was released from apoptotic cells and selectively bound to shed POS vesicles and apoptotic cells, possibly via externalized phosphatidylserine. ABCF1 is predominantly expressed in POSs and colocalized with the POS marker rhodopsin, providing geographical convenience for regulation of RPE phagocytosis. Collectively these results suggest that ABCF1 is released from and binds to shed POSs in an autocrine manner to facilitate RPE phagocytosis through a conserved pathway. Furthermore, the new approach is broadly applicable to many other phagocytes and will enable systematic elucidation of their ligands to understand extrinsic regulation and cargo recognition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.