ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2015
The inorganic anatomy of the mammalian preimplantation embryo and the requirement of zinc during the first mitotic divisions.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 42 citations in OpenAlex.
- X-ray fluorescence microscopy exposure estimates using a single excitation energy.Journal of synchrotron radiation · 2026Article
- X-ray fluorescence microscopy exposure estimates using a single excitation energy.bioRxiv : the preprint server for biology · 2026Article
- What we could not have known: ovarian cycles, follicle development, and the signature of life.Molecular human reproduction · 2026Review
- The oocyte zinc transportereLife · 2025Article
- Ambient temperature transport of human oocytes: an unexpected research resource.Journal of assisted reproduction and genetics · 2025Article
- Zinc eluted from glassware is a risk factor for embryo development in human and animal assisted reproduction†.Biology of reproduction · 2025Article
- Zinc transporter ZnT3/Slc30a3 has a potential role in zinc ion influx in mouse oocytes.The Journal of reproduction and development · 2024Article
- Review
- Phospholipase C Zeta 1 (PLCZ1): The Function and Potential for Fertility Assessment and In Vitro Embryo Production in Cattle and Horses.Veterinary sciences · 2023Review
- The role of zinc in follicular development.Molecular biology reports · 2023Review
- Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation.Animals : an open access journal from MDPI · 2022Article
- Dynamic zinc fluxes regulate meiotic progression in Caenorhabditis elegans†.Biology of reproduction · 2022Article
- Quantitative imaging approaches to understanding biological processing of metal ions.Current opinion in chemical biology · 2022Review
- Metal ion fluxes controlling amphibian fertilization.Nature chemistry · 2021Article
- Copper/Zinc Ratio Can Be a Marker to Diagnose Ectopic Pregnancy and Is Associated with the Oxidative Stress Status of Ectopic Pregnancy Cases.Biological trace element research · 2021Article
- Role of zinc in female reproduction.Biology of reproduction · 2021Review
- Article
- Zinc protection of fertilized eggs is an ancient feature of sexual reproduction in animals.PLoS biology · 2020Article
- Zinc exocytosis is sensitive to myosin light chain kinase inhibition in mouse and human eggs.Molecular human reproduction · 2020Article
- Lessons from bioengineering the ovarian follicle: a personal perspective.Reproduction (Cambridge, England) · 2019Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundZinc is the most abundant transition metal in the mammalian oocyte, and dynamic fluxes in intracellular concentration are essential for regulating both meiotic progression and fertilization. Whether the defined pathways of zinc utilization in female meiosis directly translate to mitotic cells, including the mammalian preimplantation embryo, has not been studied previously.
resultsWe determined that zinc is the most abundant transition metal in the preimplantation embryo, with levels an order of magnitude higher than those of iron or copper. Using a zinc-specific fluorescent probe, we demonstrated that labile zinc is distributed in vesicle-like structures in the cortex of cells at all stages of preimplantation embryo development. To test the importance of zinc during this period, we induced zinc insufficiency using the heavy metal chelator N,N,N',N'-tetrakis-(2-pyridylmethyl)-ethylenediamine (TPEN). Incubation of embryos in media containing TPEN resulted in a developmental arrest that was specific to zinc chelation and associated with compromised mitotic parameters. The developmental arrest due to zinc insufficiency was associated with altered chromatin structure in the blastomere nuclei and decreased global transcription.
conclusionsThese results demonstrate for the first time that the preimplantation embryo requires tight zinc regulation and homeostasis for the initial mitotic divisions of life.
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