ArticleJournal of clinical microbiology2015
Development of Conventional and Real-Time Quantitative PCR Assays for Diagnosis and Monitoring of Scabies.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- A systematic review of scabies transmission models and data to evaluate the cost-effectiveness of scabies interventions.PLoS neglected tropical diseases · 2019Pooled it
- Scabies Diagnosis: A Narrative Review of Diagnostic Methods, Clinical Misdiagnosis, and Emerging Directions.Journal of clinical medicine · 2026Review
- Moderately ectopic expressing NLP27 in potato confers high disease resistance without yield penalty.Plant cell reports · 2026Article
- A LAMP-based assay for rapid detection of human scabies using clinical skin scraping specimens.Scientific reports · 2026Article
- Article
- Exploring transmission dynamics of the Sarcoptes scabiei mite in humans by combining molecular typing and epidemiological variables, the Netherlands 2016-2023.Parasites & vectors · 2024Article
- Clinical practice guidelines for the diagnosis and treatment of scabies in Korea: Part 1. Epidemiology, clinical manifestations, and diagnosis - a secondary publication.Ewha medical journal · 2024Article
- Scabies in infants and children - a narrative review.European journal of pediatrics · 2024Review
- Review
- Health care cost of crusted scabies in Aboriginal communities in the Northern Territory, Australia.PLoS neglected tropical diseases · 2022Article
- Laboratory-based diagnosis of scabies: a review of the current status.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2022Review
- Effectiveness of CytochromeAnnals of dermatology · 2021Article
- Dermoscopy, light microscopy, and real-time polymerase chain reaction for the diagnosis of scabies. Preliminary results.Postepy dermatologii i alergologii · 2021Article
- Review
- Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis.Scientific reports · 2021Article
- Molecular diagnosis of scabies using a novel probe-based polymerase chain reaction assay targeting high-copy number repetitive sequences in the Sarcoptes scabiei genome.PLoS neglected tropical diseases · 2021Article
- The 2020 International Alliance for the Control of Scabies Consensus Criteria for the Diagnosis of Scabies.The British journal of dermatology · 2020Review
- The Management of Scabies in the 21st Century: Past, Advances and Potentials.Acta dermato-venereologica · 2020Review
- Diagnostic value of the molecular detection of Sarcoptes scabiei from a skin scraping in patients with suspected scabies.PLoS neglected tropical diseases · 2020Article
- Sarcoptes scabiei on hedgehogs in New Zealand.Parasitology research · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Scabies remains the most prevalent, endemic, and neglected ectoparasitic infestation globally and can cause institutional outbreaks. The sensitivity of routine microscopy for demonstration of Sarcoptes scabiei mites or eggs in skin scrapings is only about 50%. Except for three studies using conventional or two-tube nested PCR on a small number of cases, no systematic study has been performed to improve the laboratory diagnosis of this important infection. We developed a conventional and a real-time quantitative PCR (qPCR) assay based on the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of S. scabiei. The cox1 gene is relatively well conserved, with its sequence having no high levels of similarity to the sequences of other human skin mites, pathogenic zoonotic mites, or common house dust mite species. This mitochondrial gene is also present in large quantities in arthropod cells, potentially improving the sensitivity of a PCR-based assay. In our study, both assays were specific and were more sensitive than microscopy in diagnosing scabies, with positive and negative predictive values of 100%. The S. scabiei DNA copy number in the microscopy-positive specimens was significantly higher than that in the microscopy-negative specimens (median S. scabiei DNA copy number, 3.604 versus 2.457 log10 copies per reaction; P = 0.0213). In the patient with crusted scabies, the qPCR assay performed on lesional skin swabs instead of scrapings revealed that the parasite DNA load took about 2 weeks to become negative after treatment. The utility of using lesional skin swabs as an alternative sample for diagnosis of scabies by PCR should be further evaluated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.