ArticleBMC evolutionary biology2015
Phylogenomics of asexual Epichloë fungal endophytes forming associations with perennial ryegrass.
Article in BMC evolutionary biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Predicting Perennial Ryegrass Cultivars and the Presence of anSensors (Basel, Switzerland) · 2025Article
- Single Seed Near-Infrared Hyperspectral Imaging for Classification of Perennial Ryegrass Seed.Sensors (Basel, Switzerland) · 2023Article
- Epichloë scottii sp. nov., a new endophyte isolated from Melica uniflora is the missing ancestor of Epichloë disjuncta.IMA fungus · 2022Article
- A Metabolomic Study ofMetabolites · 2022Article
- Article
- Metabolic Potential ofMicroorganisms · 2021Review
- Global Changes in AsexualMicroorganisms · 2021Article
- The Impact of Alkaloid-ProducingToxins · 2021Review
- Genetic Diversity of the Symbiotic FungusFrontiers in microbiology · 2021Article
- Fungal Endophyte Colonization Patterns Alter Over Time in the Novel Association BetweenFrontiers in plant science · 2020Article
- Generation ofMicroorganisms · 2019Article
- Transcriptome Analysis of Choke Stroma and Asymptomatic Inflorescence Tissues Reveals Changes in Gene Expression in BothMicroorganisms · 2019Article
- Analysis of the Indole Diterpene Gene Cluster for Biosynthesis of the Epoxy-Janthitrems inMicroorganisms · 2019Article
- Genetic modification of asexual Epichloë endophytes with the perA gene for peramine biosynthesis.Molecular genetics and genomics : MGG · 2019Article
- Horizontal transfer of a ß-1,6-glucanase gene from an ancestral species of fungal endophyte to a cool-season grass host.Scientific reports · 2017Article
- Review
- Phylogenomics of asexual Epichloë fungal endophytes forming associations with perennial ryegrass.BMC evolutionary biology · 2015Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPerennial ryegrass (Lolium perenne L.) is one of the most important species for temperate pastoral agriculture, forming associations with genetically diverse groups of mutualistic fungal endophytes. However, only two taxonomic groups (E. festucae var. lolii and LpTG-2) have so far been described. In addition to these two well-characterised taxa, a third distinct group of previously unclassified perennial ryegrass-associated endophytes was identified as belonging to a putative novel taxon (or taxa) (PNT) in a previous analysis based on simple sequence repeat (SSR) marker diversity. As well as genotypic differences, distinctive alkaloid production profiles were observed for members of the PNT group.
resultsA detailed phylogenetic analysis of perennial ryegrass-associated endophytes using components of whole genome sequence data was performed using complete sequences of 7 nuclear protein-encoding genes. Three independently selected genes (encoding a DEAD/DEAH box helicase [Sbp4], a glycosyl hydrolase [family 92 protein] and a MEAB protein), none of which have been previously used for taxonomic studies of endophytes, were selected together with the frequently used 'house-keeping' genes tefA and tubB (encoding translation elongation factor 1-α and β-tubulin, respectively). In addition, an endophyte-specific gene (perA for peramine biosynthesis) and the fungal-specific MT genes for mating-type control were included. The results supported previous phylogenomic inferences for the known species, but revealed distinctive patterns of diversity for the previously unclassified endophyte strains, which were further proposed to belong to not one but two distinct novel taxa. Potential progenitor genomes for the asexual endophytes among contemporary teleomorphic (sexual Epichloë) species were also identified from the phylogenetic analysis.
conclusionsUnique taxonomic status for the PNT was confirmed through comparison of multiple nuclear gene sequences, and also supported by evidence from chemotypic diversity. Analysis of MT gene idiomorphs further supported a predicted independent origin of two distinct perennial ryegrass-associated novel taxa, designated LpTG-3 and LpTG-4, from different members of a similar founder population related to contemporary E. festucae. The analysis also provided higher resolution to the known progenitor contributions of previously characterised perennial ryegrass-associated endophyte taxa.
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