ArticleProceedings of the National Academy of Sciences of the United States of America2015
Syringyl lignin production in conifers: Proof of concept in a Pine tracheary element system.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 133 citations in OpenAlex.
- Physiological roles of lignins - tuning cell wall hygroscopy and biomechanics.The New phytologist · 2025Review
- Lignin: the primary component responsible for endocarp sclerosis in the development of Cornus officinalis fruit.BMC plant biology · 2025Article
- Factors underlying a latitudinal gradient in the S/G lignin monomer ratio in natural poplar variants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Integrative dynamics of cell wall architecture and plant growth under salt stress.Frontiers in plant science · 2025Review
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- Inducible Pluripotent Suspension Cell Cultures (iPSCs) to Study Plant Cell Differentiation.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- SCL14 Inhibits the Functions of the NAC043-MYB61 Signaling Cascade to Reduce the Lignin Content in AutotetraploidInternational journal of molecular sciences · 2023Article
- Adaptive strategies of Scots pine under shade: Increase in lignin synthesis and ecotypic variation in defense-related gene expression.Physiologia plantarum · 2022Article
- Stem traits, compartments and tree species affect fungal communities on decaying wood.Environmental microbiology · 2022Article
- Insights into the Molecular Regulation of Lignin Content in Triploid Poplar Leaves.International journal of molecular sciences · 2022Article
- Review
- QTL Identification for Stem Fiber, Strength and Rot Resistance in a DH Population from an Alien Introgression ofPlants (Basel, Switzerland) · 2022Article
- Localised laccase activity modulates distribution of lignin polymers in gymnosperm compression wood.The New phytologist · 2021Article
- Comparison of methodologies used to determine aromatic lignin unit ratios in lignocellulosic biomass.Biotechnology for biofuels · 2021Article
- BEL1-like Homeodomain Protein BLH6a Is a Negative Regulator ofFrontiers in plant science · 2021Article
- Genome-wide analysis of general phenylpropanoid and monolignol-specific metabolism genes in sugarcane.Functional & integrative genomics · 2021Article
- Application of compound material alleviates saline and alkaline stress in cotton leaves through regulation of the transcriptome.BMC plant biology · 2020Article
- Discovering Biomass Structural Determinants Defining the Properties of Plant-Derived Renewable Carbon Fiber.iScience · 2020Article
- Transcriptional analysis of arogenate dehydratase genes identifies a link between phenylalanine biosynthesis and lignin biosynthesis.Journal of experimental botany · 2020Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conifers (softwoods) naturally lack syringyl units in their lignins, rendering lignocellulosic materials from such species more difficult to process than syringyl-rich hardwood species. Using a transformable Pinus radiata tracheary element (TE) system as an experimental platform, we investigated whether metabolic engineering can be used to create syringyl lignin in conifers. Pyrolysis-GC/MS and 2D-NMR analysis of P. radiata TE cultures transformed to express ferulate 5-hydroxylase (F5H) and caffeic acid O-methyltransferase (COMT) from Liquidambar styraciflua confirmed the production and incorporation of sinapyl alcohol into the lignin polymer. Transformation with F5H was sufficient for the production of syringyl lignin in TEs, but cotransformation with COMT improved its formation. In addition, lower levels of the pathway intermediate 5-hydroxyconiferyl alcohol were evidenced in cotransformation experiments, indicating that the introduction of the COMT overcame the inefficiency of the native pine methyltransferases for supporting sinapyl alcohol production.Our results provide the proof of concept that it is possible to generate a lignin polymer that contains syringyl units in softwood species such as P. radiata, suggesting that it might be possible to retain the outstanding fiber properties of softwoods while imbuing them with the lignin characteristics of hardwoods that are more favorable for industrial processing.
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