ArticleBiophysical journal2015
The mechanical properties of early Drosophila embryos measured by high-speed video microrheology.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Adherens junctions as molecular regulators of emergent tissue mechanics.Nature reviews. Molecular cell biology · 2024Review
- Reshaping the Syncytial Drosophila Embryo with Cortical Actin Networks: Four Main Steps of Early Development.Results and problems in cell differentiation · 2024Review
- Fly embryo nuclei riding on two-fluid flow.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Membrane-actin interactions in morphogenesis: Lessons learned from Drosophila cellularization.Seminars in cell & developmental biology · 2023Review
- Two-step nuclear centring by competing microtubule- and actin-based mechanisms in 2-cell mouse embryos.EMBO reports · 2022Article
- Foregut organ progenitors and their niche display distinct viscoelastic properties in vivo during early morphogenesis stages.Communications biology · 2022Article
- Article
- Force measurements of Myosin II waves at the yolk surface during Drosophila dorsal closure.Biophysical journal · 2022Article
- Article
- Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos.Journal of visualized experiments : JoVE · 2020Article
- Viscoelastic voyages - Biophysical perspectives on cell intercalation during Drosophila gastrulation.Seminars in cell & developmental biology · 2020Review
- Exfoliated near infrared fluorescent silicate nanosheets for (bio)photonics.Nature communications · 2020Article
- The mechanobiology of actin cytoskeletal proteins during cell-cell fusion.Journal of the Royal Society, Interface · 2019Article
- Mechanical Model of Nuclei Ordering in Drosophila Embryos Reveals Dilution of Stochastic Forces.Biophysical journal · 2018Article
- Spatial regulation of contractility by Neuralized and Bearded during furrow invagination in Drosophila.Nature communications · 2017Article
- Evaluating biomechanical properties of murine embryos using Brillouin microscopy and optical coherence tomography.Journal of biomedical optics · 2017Article
- Relieving the Pressure on Tissue Development.Biophysical journal · 2017Article
- Microfluidic analysis of oocyte and embryo biomechanical properties to improve outcomes in assisted reproductive technologies.Molecular human reproduction · 2017Review
- A toolbox to explore the mechanics of living embryonic tissues.Seminars in cell & developmental biology · 2016Review
- Local dynamic mechanical analysis for heterogeneous soft matter using ferrule-top indentation.Soft matter · 2016Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In early development, Drosophila melanogaster embryos form a syncytium, i.e., multiplying nuclei are not yet separated by cell membranes, but are interconnected by cytoskeletal polymer networks consisting of actin and microtubules. Between division cycles 9 and 13, nuclei and cytoskeleton form a two-dimensional cortical layer. To probe the mechanical properties and dynamics of this self-organizing pre-tissue, we measured shear moduli in the embryo by high-speed video microrheology. We recorded position fluctuations of injected micron-sized fluorescent beads with kHz sampling frequencies and characterized the viscoelasticity of the embryo in different locations. Thermal fluctuations dominated over nonequilibrium activity for frequencies between 0.3 and 1000 Hz. Between the nuclear layer and the yolk, the cytoplasm was homogeneous and viscously dominated, with a viscosity three orders of magnitude higher than that of water. Within the nuclear layer we found an increase of the elastic and viscous moduli consistent with an increased microtubule density. Drug-interference experiments showed that microtubules contribute to the measured viscoelasticity inside the embryo whereas actin only plays a minor role in the regions outside of the actin caps that are closely associated with the nuclei. Measurements at different stages of the nuclear division cycle showed little variation.
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